Product Pathways - Protein Stability
RanGAP1 (D2T7T) Rabbit mAb #36067
|36067S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|36067||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,Mouse,Rat,Monkey,||Endogenous||68, 82||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
RanGAP1 (D2T7T) Rabbit mAb recognizes endogenous levels of total RanGAP1 protein. This antibody detects unmodified and SUMO-1 modified RanGAP1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RanGAP1 protein.
Western blot analysis of extracts from various cell lines using RanGAP1 (D2T7T) Rabbit mAb.
Ran GTPase activating protein 1 (RanGAP1) regulates GTP hydrolysis by Ran, which is required for the ability of Ran to regulate nucleocytoplasmic shuttling (1). A significant fraction of cellular RanGAP1 is covalently modified by SUMO-1, which is required for relocalization of RanGAP1from the cytoplasm to the nuclear pore complex and the mitotic spindle (2-5). Research studies demonstrate that RanGAP1 sumoylation is required for stable association of RanGAP1 with RanBP2. Together with the SUMO-conjugating enzyme UBC9/UBE2I, RanGAP1 and RanBP2 are part of a SUMO E3 ligase complex that is implicated in regulating nucleocytoplasmic protein trafficking (6-8). Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity (9,10).
- Bischoff, F.R. et al. (1994) Proc Natl Acad Sci U S A 91, 2587-91.
- Mahajan, R. et al. (1997) Cell 88, 97-107.
- Matunis, M.J. et al. (1998) J Cell Biol 140, 499-509.
- Matunis, M.J. et al. (1996) J Cell Biol 135, 1457-70.
- Joseph, J. et al. (2002) J Cell Biol 156, 595-602.
- Hutten, S. et al. (2008) Mol Biol Cell 19, 2300-10.
- Werner, A. et al. (2012) Mol Cell 46, 287-98.
- Zhu, S. et al. (2009) Mol Cell 33, 570-80.
- Swaminathan, S. et al. (2004) J Cell Biol 164, 965-71.
- Takeda, E. et al. (2005) Cell Struct Funct 30, 69-80.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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