Cell Signaling Technology

Product Pathways - Metabolism

SNAT1/SLC38A1 (D9L2P) Rabbit mAb #36057

No. Size Price
36057S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
36057 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50, 70 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

SNAT1/SLC38A1 (D9L2P) Rabbit mAb recognizes endogenous levels of total SNAT1/SLC38A1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly37 of human SNAT1/SLC38A1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various brain samples and cell lines using SNAT1/SLC38A1 (D9L2P) Rabbit mAb.

IP

IP

Immunoprecipitaion of SNAT1/SLC38A1 from BT-549 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SNAT1/SLC38A1 (D9L2P) Rabbit mAb. Western blot analysis was performed using SNAT1/SLC38A1 (D9L2P) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678.

Background

SNAT1/SLC38A1 belongs to the system A transporters that mediate Na+-dependent transport of short-chain neutral amino acids such as alanine, serine, and glutamine. SNAT1

/SLC38A1 mediates the uptake of glutamine in neurons and plays a crucial role in glutamate-glutamine cycle. Steep concentration gradients across the plasma membrane are achieved by coupling of the electrochemical sodium gradient to amino acid transport. This allows a unidirectional mode of transport for SNAT1/SLC38A1. Upregulation of SNAT1/SLC38A1 by neurotrophic factors is key to dendritic growth and branching of cortical neurons. High expression of SNAT1/SLC38A1 is found in cerebral cortex primarily in neurons and to a lesser extent in astrocytes (1-4). Elevated SNAT1/SLC38A1 expression is prominent in human solid tumors including gliomas, hepatocellular carcinomas and human breast cancer (5-8). Research studies show that an aberrant SNAT1

/SLC38A1 expression profile correlates with solid tumor recurrence and poor prognosis in patients with cholangiocarcinoma (9).

  1. Yao, D. et al. (2000) J Biol Chem 275, 22790-7.
  2. Mackenzie, B. et al. (2003) J Biol Chem 278, 23720-30.
  3. Chaudhry, F.A. et al. (2002) J Cell Biol 157, 349-55.
  4. Yu, W.L. et al. (2011) J Surg Res 171, 663-8.
  5. Melone, M. et al. (2004) Cereb Cortex 14, 562-74.
  6. Kondoh, N. et al. (2007) Int J Oncol 31, 81-7.
  7. Sidoryk, M. et al. (2004) Neuroreport 15, 575-8.
  8. Wang, K. et al. (2013) BMC Cancer 13, 343.
  9. Burkhalter, J. et al. (2007) J Biol Chem 282, 5152-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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