Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

FLI1 (D7N5M) Rabbit mAb #35980

ERGB   Friend leukemia intregration  

No. Size Price
35980S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
35980 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 52 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat, Zebrafish,

Specificity / Sensitivity

FLI1 (D7N5M) Rabbit mAb recognizes endogenous levels of total FLI1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of human FLI1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FLI1 (D7N5M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of FLI1 from OCI-AML3 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype control #3900, and lane 3 is FLI1 (D7N5M) Rabbit mAb. Western blot analysis was performed using FLI1 (D7N5M) Rabbit mAb.


Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the Fli1 locus is a common retroviral insertion site for the Friend murine leukemia virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing's sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing’s sarcoma, an aggressive bone and soft tissue tumor (8,9).

  1. Spyropoulos, D.D. et al. (2000) Mol Cell Biol 20, 5643-52.
  2. Pimanda, J.E. et al. (2007) Proc Natl Acad Sci U S A 104, 17692-7.
  3. Jagadeeswaran, P. et al. (2010) Blood Cells Mol Dis 44, 175-80.
  4. Tijssen, M.R. et al. (2011) Dev Cell 20, 597-609.
  5. Ben-David, Y. et al. (1990) Proc Natl Acad Sci U S A 87, 1332-6.
  6. Kornblau, S.M. et al. (2011) Blood 118, 5604-12.
  7. May, W.A. et al. (1993) Proc Natl Acad Sci U S A 90, 5752-6.
  8. Braun, B.S. et al. (1995) Mol Cell Biol 15, 4623-30.
  9. Riggi, N. and Stamenkovic, I. (2007) Cancer Lett 254, 1-10.

Application References

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