Cell Signaling Technology

Product Pathways - Development

Nanog Antibody #3580


No. Size Price
3580S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
3580 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 42 Rabbit
F 1:400
IF-IC 1:800
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

Nanog Antibody detects endogenous levels of total nanog protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence at the N-terminus of human nanog. Antibodies are purified by Protein A and peptide affinity chromatography.

多克隆抗体是通过一种合成的肽段去免疫动物产生。该肽段与人nanog蛋白邻近氨基末端的氨基酸序列一致。抗体由蛋白A和肽段亲和层析技术纯化 。

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and NCCIT cells using Nanog Antibody.使用Nanog抗体对NTERA2和NCCIT细胞提取物进行western blot分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Nanog Antibody.使用Nanog抗体对HeLa细胞(蓝色)和NTERA2细胞(绿色)进行流式细胞分析。



Confocal immunofluorescent analysis of NTERA2 cells (left) and HeLa cells (right) using Nanog Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用Nanog 抗体(绿色)对NTERA2(左)和HeLa细胞(右)进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554 鬼笔环肽(红色)标记。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 20 μl of Nanog Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞与20μl Nanog抗体或2μl正常兔IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人Oct-4启动子引物#4641,SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得。


Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

Nanog是一个含有同源结构域的转录因子,对于维持胚胎干细胞的多潜能性和自我更新非常重要(1)。Nanog的表达由包括Sox2和关键多潜能性调控因子Oct-4在内的调控网络调控(1)。近来对于体细胞重编程的研究进展主要通过整合包含nanog, Oct-4, Sox2, KLF4, c-Myc和 LIN28等转录因子病毒表达来实现 (2,3).

  1. Kim, J. et al. (2008) Cell 132, 1049-61.
  2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
  3. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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