Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-CD19 (Tyr531) Antibody #3571


No. Size Price
3571S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3571T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3571 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 95 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-CD19 (Tyr531) Antibody detects endogenous levels of CD19 only when phosphorylated at tyrosine 531.

Phospho-CD19 (Tyr531) Antibody 只能检测内源的在tyr531位点磷酸化的CD19蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr531 of human CD19. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的CD19 Tyr531位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of SDS extracts from untreated or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells using Phospho-CD19 (Tyr531) Antibody (upper) and control CD19 Antibody #3574 (lower).Western免疫印迹。用Phospho-CD19 (Tyr531) Antibody (上图)和 control CD19 Antibody #3574 (下图)研究未经处理的和经anti-human IgM treated (12 µg/ml,2 min) 处理的Ramos细胞的SDS细胞提取液。


CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).

CD19是一个分子量为95 kDa的共受体, 它能在B细胞中放大级联反应的信号(1)。在B细胞的表面, CD19与CD21, CD81和 Leu-13相连并发挥其作用。CD19的细胞质尾巴有9个保守的酪氨酸在CD19介导的偶联信号分子到受体时发挥了重要的作用(1)。B 细胞受体或CD19连结后, CD19的Tyr531和Tyr500位点被逐渐的磷酸化。此磷酸化使得PI3激酶和Src家族的酪氨酸激酶偶联到CD19并激活PI3K和Src 信号通路(2,3)。B细胞受体与CD19的共结合也促进了CD19中Tyr409位点的磷酸化。在此位点的磷酸化也使得它能够结合到Vav并介导提高胞内钙响应和JNK信号通路(4,5)。

  1. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
  2. Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46.
  3. Fujimoto, M. et al. (2000) Immunity 13, 47-57.
  4. O'Rourke, L.M. et al. (1998) Immunity 8, 635-45.
  5. Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :