Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb #3556

No. Size Price
3556S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3556 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 90 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb detects endogenous levels of RSK2 only when phosphorylated at Ser227. It shows cross-reactivity with RSK1 when phosphorylated at the homologous serine residues.

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb兔单抗能检测内源性Ser227位点磷酸化的RSK2蛋白水平。该抗体也能与同源丝氨酸残基磷酸化的RSK1蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser227 of human RSK2.

该单克隆抗体通过使用与人源RSK2蛋白Ser227位点周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, COS and NIH/3T3 cells, treated with lambda-phosphatase or TPA/UV, using Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb.Western blot方法检测细胞提取物:lambda磷酸酶和TPA/UV处理的COS, NIH/3T3, HeLa细胞。使用的抗体是Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb。

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族,其特征是包含2个不同的功能性激酶结构域(1)和一个羧基端的细胞外信号调节激酶(ERKs)结合位点(2)。RSK激酶结构域内或以外的一些位点,包括Ser380, Thr359, Ser363和Thr573,对于激酶的激活非常重要(3)。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下,通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活(3)。

PDK1 phosphorylates Ser227 in the activation loop of the amino-terminal kinase domain of RSK2, leading to substantial activation of the kinase in vitro and in vivo. The constitutively active PDK1 cooperates with ERK in the activation of RSK following the exposure of cells to growth factors or UV-light (4, 5).

在体内及体外,PDK1都能磷酸化RSK2胺端激酶区域内活化环的Ser227位点,导致激酶的大量活化。在经过生长因子或UV处理的细胞中,组成型活化的PDK1与ERK协同作用,激活RSK(4, 5)。

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  4. Jensen, C.J. et al. (1999) J Biol Chem 274, 27168-76.
  5. Mérienne, K. et al. (2000) Oncogene 19, 4221-9.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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