Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-c-Cbl (Tyr774) Antibody #3555

No. Size Price
3555S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3555T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3555 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 120 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-c-Cbl(Tyr774)抗体识别内源性的Tyr774磷酸化的c-Cbl蛋白,该抗体不与其他相关酪氨酸磷酸化蛋白发生交叉反应。

Source / Purification

此抗体是通过合成与人c-Cbl蛋白Tyr774邻近的氨基酸残基序列一致的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from 293-T cells transfected with c-Cbl and Fyn (lanes 3 and 5) or without Fyn (lanes 2 and 4), untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-c-Cbl (Tyr774) Antibody. (Lysates provided by Dr. A.L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.) 使用Phospho-c-Cbl (Tyr774)抗体对转染c-Cbl和Fyn(泳道3和5)后、无Fyn转染(泳道2和4)并未经处理或经小牛肠碱性磷酸酶(CIP)处理后的293-T细胞提取物中磷酸化c-Cbl(Tyr775)进行检测的免疫印迹分析结果(裂解物由哈佛大学AL Reddi博士提供)。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293-T cells transfected with c-Cbl and Fyn (lanes 3 and 5) or without Fyn (lanes 2 and 4), untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-c-Cbl (Tyr774) Antibody. (Lysates provided by Dr. A.L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.)

Background

c-Cbl原癌基因是一种广泛表达的细胞质衔接蛋白,主要表达于造血细胞(1,2)。对于许多种细胞表面受体的刺激,c-Cbl迅速地进行酪氨酸磷酸化,并与许多细胞内信号分子例如蛋白酪氨酸激酶,磷脂酰肌醇-3激酶,Crk和14-3-3蛋白相联合(3,4)。c-Cbl具有高度保守的氨基末端磷酸酪氨酸结合结构域(TKB),以及C3HC4 RING指模序。TKB识别活化的酪氨酸激酶受体(RTKs)上的磷酸化的酪氨酸,以及其他非受体酪氨酸激酶。环指模序募集泛素缀合酶。这两种结构域主要负责c-Cbl的泛素连接酶活性,以及RTKs的下调(3)。研究表明人癌症组织,c-Cbl以一种肿瘤特异的方式频繁地进行酪氨酸磷酸化(5)。c-Cbl Tyr731的磷酸化为下游的信号组分诸如p85 和 Fyn提供了一个停泊位点(6)。

  1. Blake, T.J. et al. (1991) Oncogene 6, 653-657.
  2. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
  3. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307.
  4. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331.
  5. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
  6. Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications