Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3β (Thr390) Antibody #3548

EC   Glycogen synthase kinase-3 beta   GSK-3 beta   GSK-3beta  

No. Size Price
3548S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3548 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 46 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-GSK-3β (Thr390) Antibody detects endogenous levels of human GSK-3β protein only when phosphorylated at Thr390.

Phospho-GSK-3β (Thr390) Antibody可以检测出在Thr390位点磷酸化的内源性总GSK-3β蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr390 of human GSK-3β. Antibodies are purified by peptide affinity chromatography.


Western blot analysis of extracts from HeLa cells, either hydroxyurea-treated (4 mM, 20 hrs) to induce G1/S phase arrest or paclitaxel-treated (100 nM/ml, 20 hrs) for G2/M phase arrest, using Phospho-GSK-3β (Thr390) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower) to show induction of phospho-GSK and equal loading.

Western blot分析Hela细胞提取物,羟基脲(4 mM, 20 hrs)处理组诱导G1/S 期阻滞,紫杉醇处理组(100 nM/ml, 20 hrs)诱导G2/M阻滞,所用抗体为Phospho-GSK-3β (Thr390) Antibody (上) 和 GSK-3β (27C10) Rabbit mAb 兔单抗#9315 (下) 用以显示phospho-GSK的诱导和相同上样量.


Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Phosphorylation of Thr390 on GSK-3β was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery (6). The phosphorylation of this site was also reported by several other labs using phosphoproteomic analysis on mitotic cell extracts (7-10).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  6. Thornton, T.M. et al. (2008) Science 320, 667-70.
  7. Daub, H. et al. (2008) Mol Cell 31, 438-48.
  8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
  9. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
  10. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.
  11. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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