Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3β (Thr390) Antibody #3548

EC   Glycogen synthase kinase-3 beta   GSK-3 beta   GSK-3beta  

No. Size Price
3548S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3548 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 46 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-GSK-3β (Thr390) Antibody detects endogenous levels of human GSK-3β protein only when phosphorylated at Thr390.

Phospho-GSK-3β (Thr390) Antibody可以检测出在Thr390位点磷酸化的内源性总GSK-3β蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr390 of human GSK-3β. Antibodies are purified by peptide affinity chromatography.


Western blot analysis of extracts from HeLa cells, either hydroxyurea-treated (4 mM, 20 hrs) to induce G1/S phase arrest or paclitaxel-treated (100 nM/ml, 20 hrs) for G2/M phase arrest, using Phospho-GSK-3β (Thr390) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower) to show induction of phospho-GSK and equal loading.

Western blot分析Hela细胞提取物,羟基脲(4 mM, 20 hrs)处理组诱导G1/S 期阻滞,紫杉醇处理组(100 nM/ml, 20 hrs)诱导G2/M阻滞,所用抗体为Phospho-GSK-3β (Thr390) Antibody (上) 和 GSK-3β (27C10) Rabbit mAb 兔单抗#9315 (下) 用以显示phospho-GSK的诱导和相同上样量.


Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Phosphorylation of Thr390 on GSK-3β was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery (6). The phosphorylation of this site was also reported by several other labs using phosphoproteomic analysis on mitotic cell extracts (7-10).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  6. Thornton, T.M. et al. (2008) Science 320, 667-70.
  7. Daub, H. et al. (2008) Mol Cell 31, 438-48.
  8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
  9. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
  10. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.
  11. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :