Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-NPM (Thr199) Antibody #3541

B23   NO38   nucleophosmin   numatrin  

No. Size Price
3541S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
3541 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 38 Rabbit
IP 1:100
IHC-P 1:200
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-NPM (Thr199) Antibody detects endogenous levels of NPM only when phosphorylated at threonine 199. Phospho-NPM (Thr199) Antibody 能够检测内源性苏氨酸(199位)磷酸化的NPM蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr199 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对苏氨酸(199位)的磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using Phospho-NPM (Thr199) Antibody (upper) or NPM Antibody #3542 (lower). western blot方法检测细胞周期不同阶段同步化的HeLa细胞提取物,使用的抗体为 Phospho-NPM (Thr199) Antibody (上图) 或NPM Antibody #3542 (下图).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-NPM (Thr199) Antibody. 免疫组织化学方法检测石蜡包埋人类乳腺癌组织,显示核定位,使用的抗体为Phospho-NPM(Thr199) Antibody。



Confocal immunofluorescent analyis of HT-29 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-NPM (Thr199) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光方法检测HT-29 细胞, 未处理 (左图) 和λ 磷酸酶处理(右图), 检测抗体为Phospho-NPM (Thr199) Antibody ,呈绿色。肌动蛋白纤维由DY-554 鬼笔环肽标记,呈红色。蓝色伪彩为DNA荧光染料(产品信息为 DRAQ5®#4084 )。


Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4). 核磷蛋白(NPM;又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能,包括核糖体组装和运输,胞质/胞核运输,DNA聚合酶α活性的调节,中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化,最终激活融合激酶(3,4)。

NPM associates with unduplicated centrosomes and is a direct substrate of Cdk2-cyclin E in centrosome duplication (4). Upon phosphorylation at Thr199 by Cdk2-cyclin E, NPM dissociates from centrosomes, and this dissociation is a prerequisite step for centrosome to initiate duplication (5). NPM与未复制的中心体结合,在中心体复制过程中是Cdk2-cyclin E的一个直接底物(4)。Cdk2-cyclin E磷酸化NPM苏氨酸199位后,NPM与中心体解聚,这是中心体复制起始的前提(5)。

  1. Okuda, M. et al. (2000) Cell 103, 127-140.
  2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
  3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
  4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.
  5. Tokuyama, Y. et al. (2001) J. Biol. Chem. 276, 21529-21537.

Application References

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