Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Btk (Ser180) (3D3) Mouse mAb #3537

Bruton''s tyrosine kinase   Btk   Itk   Tec  

No. Size Price
3537S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3537 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-Btk (Ser180) (3D3) Mouse mAb detects transfected levels of Btk protein when phosphorylated at Ser180. For Western immunoblotting, CST recommends using immunoprecipitated Btk first.

Phospho-Btk (Ser180) (3D3) Mouse mAb鼠单抗只能检测转染的在Ser180位点磷酸化的Btk蛋白。对Western blot,CST推荐首先免疫共沉淀Btk蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser180 of human Btk.

此单克隆抗体是通过合成人源对应的Btk Ser180位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from hydrogen peroxide treated or serum starved THP1 cells using Phospho-Btk (Ser180) (3D3) Mouse mAb.Western免疫印迹。用Phospho-Btk (Ser180) (3D3) Mouse mAb研究经H2O2处理或血清饥饿的THP1细胞的细胞提取液。

Background

Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity (8).

Bruton's 酪氨酸激酶 (Btk)是细胞质激酶Btk/Tec家族的一员。与Btk家族的其它成员一样,它包含了血小板-白细胞C激酶底物同系(PH) 区域和,Src同系的SH3 和 SH2 区域。Btk在 B 细胞的发育过程中起了重要的作用 (1,2)。 B 细胞通过各种配体的激活总是伴随着PH 区域结合到磷脂酰肌醇-3,4,5-三磷酸化介导的Btk蛋白向膜定位 (3-5)。Btk定位于细胞膜后具有活性,并瞬间磷酸化两个酪氨酸残基Tyr551 和 Tyr223。位于活性环上的Tyr551位点被Src家族酪氨酸激酶转磷酸化,导致了SH3区域 Tyr223 位点的自磷酸化, 这对B 细胞的完全激活非常重要(6,7)。PKCβ通过磷酸化Btk的Ser180位点而负调控Btk的激活,并导致了细胞膜招募能力的降低,磷酸根的转移和接下来的激活(8)。The PKC 的抑制信号看起来是B细胞受体信号保持Btk最合适活性的中的重要决定因素(8)。

  1. Khan, W.N. (2001) Immunol Res 23, 147-56.
  2. Lewis, C.M. et al. (2001) Curr Opin Immunol 13, 317-25.
  3. Salim, K. et al. (1996) EMBO J 15, 6241-50.
  4. Rameh, L.E. et al. (1997) J Biol Chem 272, 22059-66.
  5. Várnai, P. et al. (1999) J Biol Chem 274, 10983-9.
  6. Rawlings, D.J. et al. (1996) Science 271, 822-5.
  7. Park, H. et al. (1996) Immunity 4, 515-25.
  8. Kang, S.W. et al. (2001) EMBO J 20, 5692-702.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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