Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-PNK1 (Ser114/Thr118) Antibody #3522

No. Size Price
3522S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3522 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 59 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PNK1 (Ser114/Thr118) Antibody detects endogenous levels of PNK1 only when phosphorylated on Ser114 and Thr118.Phospho-PNK1 (Ser114/Thr118) Antibody能够识别内源性丝氨酸(114位)苏氨酸(118位)PNK1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser114 and Thr118 of human PNK1. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体是由合成的人源的针对PNK1蛋白丝氨酸(114位)苏氨酸(118位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts of 293 and HeLa cells, either untreated or treated with UV radiation, using Phospho-PNK1 (Ser114/Thr118) Antibody (upper). β-actin Antibody #4967 (lower) was used to control for loading.Western blot检测293和Hela细胞提取物,分为未处理组或紫外辐射组,使用的抗体为 Phospho-PNK1 (Ser114/Thr118) Antibody (上图)。β-actin Antibody #4967作为内对照。

Background

PNK (polynucleotide kinase) is a DNA repair enzyme that participates in single strand break repair and non-homologous end rejoining (NHEJ) for double strand breaks. PNK possesses a 5'-DNA kinase activity and a 3'-DNA phosphatase activity (1,2). It has three domains, a C-terminal kinase domain, a central phosphatase domain, and an N-terminal forkhead associated (FHA) domain that is responsible for protein-protein interactions. Reduction in expression of PNK by RNAi sensitizes cells to ionizing radiation and topoisomerase I inhibitors (3) PNK (多核苷酸激酶)是一种DNA修复酶,它参与单链断裂修复和双链断裂的非同源末端重组。PNK拥有5'-DNA激酶活性和3'-DNA磷酸酶活性(1,2)。它有三个结构域:一个羧基末端激酶结构域,一个中心的磷酸酶结构域,一个负责蛋白-蛋白相互作用的氨基末端叉头相关(FHA)结构域。RNAi引起的PNK1表达降低使细胞对离子辐射和拓扑异构酶抑制剂敏感(3)。

Phosphorylation of PNK1 at Ser114/ Thr118 was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PNK1 at Ser114/ Thr118 was observed in extracts of various cell lines following UV treatment. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org. 通过采用Cell Signaling Technology (CST)的PhosphoScan®和MS/MS磷酸化位点发现平台,PNK1的第114位丝氨酸和第118位苏氨酸磷酸化分别被鉴定。我们在紫外处理后的多种细胞提取物中发现了PNK1的第114位丝氨酸和第118位苏氨酸磷酸化。更多信息请访问PhosphoSitePlus®和CST的修饰位点数据库(www.phosphosite.org)。

  1. Jilani, A. et al. (1999) J Biol Chem 274, 24176-86.
  2. Dobson, C.J. and Allinson, S.L. (2006) Nucleic Acids Res 34, 2230-7.
  3. Bernstein, N.K. et al. (2008) Anticancer Agents Med Chem 8, 358-67.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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