Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-PNK1 (Ser114/Thr118) Antibody #3522

No. Size Price
3522S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3522 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 59 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PNK1 (Ser114/Thr118) Antibody detects endogenous levels of PNK1 only when phosphorylated on Ser114 and Thr118.Phospho-PNK1 (Ser114/Thr118) Antibody能够识别内源性丝氨酸(114位)苏氨酸(118位)PNK1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser114 and Thr118 of human PNK1. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体是由合成的人源的针对PNK1蛋白丝氨酸(114位)苏氨酸(118位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts of 293 and HeLa cells, either untreated or treated with UV radiation, using Phospho-PNK1 (Ser114/Thr118) Antibody (upper). β-actin Antibody #4967 (lower) was used to control for loading.Western blot检测293和Hela细胞提取物,分为未处理组或紫外辐射组,使用的抗体为 Phospho-PNK1 (Ser114/Thr118) Antibody (上图)。β-actin Antibody #4967作为内对照。


PNK (polynucleotide kinase) is a DNA repair enzyme that participates in single strand break repair and non-homologous end rejoining (NHEJ) for double strand breaks. PNK possesses a 5'-DNA kinase activity and a 3'-DNA phosphatase activity (1,2). It has three domains, a C-terminal kinase domain, a central phosphatase domain, and an N-terminal forkhead associated (FHA) domain that is responsible for protein-protein interactions. Reduction in expression of PNK by RNAi sensitizes cells to ionizing radiation and topoisomerase I inhibitors (3) PNK (多核苷酸激酶)是一种DNA修复酶,它参与单链断裂修复和双链断裂的非同源末端重组。PNK拥有5'-DNA激酶活性和3'-DNA磷酸酶活性(1,2)。它有三个结构域:一个羧基末端激酶结构域,一个中心的磷酸酶结构域,一个负责蛋白-蛋白相互作用的氨基末端叉头相关(FHA)结构域。RNAi引起的PNK1表达降低使细胞对离子辐射和拓扑异构酶抑制剂敏感(3)。

Phosphorylation of PNK1 at Ser114/ Thr118 was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PNK1 at Ser114/ Thr118 was observed in extracts of various cell lines following UV treatment. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org. 通过采用Cell Signaling Technology (CST)的PhosphoScan®和MS/MS磷酸化位点发现平台,PNK1的第114位丝氨酸和第118位苏氨酸磷酸化分别被鉴定。我们在紫外处理后的多种细胞提取物中发现了PNK1的第114位丝氨酸和第118位苏氨酸磷酸化。更多信息请访问PhosphoSitePlus®和CST的修饰位点数据库(www.phosphosite.org)。

  1. Jilani, A. et al. (1999) J Biol Chem 274, 24176-86.
  2. Dobson, C.J. and Allinson, S.L. (2006) Nucleic Acids Res 34, 2230-7.
  3. Bernstein, N.K. et al. (2008) Anticancer Agents Med Chem 8, 358-67.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :