Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-MDM2 (Ser166) Antibody #3521

No. Size Price
3521S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3521 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 90 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MDM2 (Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Ser166. Phospho-MDM2 (Ser166) Antibody能够检测内源性丝氨酸(166位)磷酸化的MDM2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对MDM2蛋白丝氨酸(166位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with Wild-type or mutant (S166S186A) MDM2, using Phospho-MDM2 (Ser166) Antibody. western blot方法检测未转染和转染野生型或突变型 (S166S186A) MDM2的COS细胞提取物,使用的抗体为Phospho-MDM2 (Ser166) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7 cells treated with IGF-1 for the indicated times, using Phospho-MDM2 (Ser166) Antibody. western blot方法检测用IGF-1于不同时间点处理MCF-7细胞提取物,使用的抗体为Phospho-MDM2 (Ser166) Antibody。

Background

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3). MDM2,一种p53泛素连接酶,在p53稳定性的调节中至关重要(1)。Akt介导的MDM2苏氨酸166和168位磷酸化增加了它和p300的相互作用,允许MDM2介导的泛素化和p53降解(2-4)。MDM2的磷酸化也阻碍其结合p19ARF,促进p53降解(3)。

  1. Haupt, Y. et al. (1997) Nature 387, 296-299.
  2. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
  3. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
  4. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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