Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520

No. Size Price
3520S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
3520T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
3520 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 38 Rabbit IgG
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Bovine, Dog,

Specificity / Sensitivity

Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb detects endogenous levels of NPM only when phosphorylated at Ser4. Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb 能够检测内源性丝氨酸(4位)磷酸化的NPM蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser4 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对NPM蛋白丝氨酸(4位点)的磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa cells, either untreated or nocodazole-treated, using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb (upper). Lower panel shows total NPM using NPM Antibody #3542. western blot方法检测未处理和nocodazole处理的HeLa细胞提取物,使用的抗体为Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb (上图)。下图示NPM总蛋白,使用的抗体为NPM Antibody #3542。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb compared to Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4). 核磷蛋白(NPM;又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能,包括核糖体组装和运输,胞质/胞核运输,DNA聚合酶α活性的调节,中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化,最终激活融合激酶(3,4)。

  1. Okuda, M. et al. (2000) Cell 103, 127-140.
  2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
  3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
  4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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