Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-NPM (Thr95) Antibody #3517

No. Size Price
3517S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3517 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 38 Rabbit
IP 1:100
F 1:200
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-NPM (Thr95) Antibody detects endogenous levels of NPM only when phosphorylated at Thr95. Phospho-NPM (Thr95) Antibody 能够检测内源性苏氨酸(95位)磷酸化的NPM蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr95 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对NPM蛋白苏氨酸(95位)的磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa cells, untreated or nocodazole-treated, using Phospho-NPM (Thr95) Antibody (upper) or NPM Antibody #3542 (lower). western blot方法检测未处理和nocodazole处理的HeLa细胞提取物,使用的抗体为Phospho-NPM (Thr95) Antibody (上图和NPM Antibody #3542(下图)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells untreated (left) and λ phosphatase-treated (right), using NPM (Thr95) phosphate Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 激光共聚焦免疫荧光方法检测HeLa细胞, 未处理 (左图) 和λ 磷酸酶处理(右图), 检测抗体为NPM (Thr95) phosphate Antibody ,呈绿色。肌动蛋白纤维由DY-554 鬼笔环肽标记,呈红色。蓝色伪彩为DNA荧光染料(产品信息为 DRAQ5®#4084 )。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-NPM (Thr95) Antibody versus propidium iodide (DNA content). The box indicates phospho-NPM positive cells. 流式细胞术检测Jurkat细胞,使用的抗体为 Phospho-NPM (Thr95) Antibody ,PI染色法检测DNA含量作为对照。框内的细胞群为 Phospho-NPM 阳性细胞。

Background

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4). 核磷蛋白(NPM;又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能,包括核糖体组装和运输,胞质/胞核运输,DNA聚合酶α活性的调节,中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化,最终激活融合激酶(3,4)。

  1. Okuda, M. et al. (2000) Cell 103, 127-140.
  2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
  3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
  4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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