Cell Signaling Technology

Product Pathways - Protein Translation

Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb #3505

PAB1   PABP1   PABPC1   PABPC2  

No. Size Price
3505S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3505 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 71 Rabbit IgG
IP 1:25
IHC-P 1:1000
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Horse,

Specificity / Sensitivity

Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb detects endogenous levels of PABP1 only when methylated on Arg455 or Arg460.

Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb兔单抗检测出仅当PABP1蛋白在Arg455或Arg460位点发生甲基化时的内源PABP1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated.

通过人工合成人源PABP1蛋白在Arg455和Arg460位点发生甲基化的多肽片段,使用该片段去免疫动物从而制备出单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb. As expected, PABP1 methylation is decreased in CARM1 knockout (-/-) mouse embryo fibroblasts (MEFs).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Methyl-PABP1 (Arg455/Arg/460) (C60A10) Rabbit mAb in the presence of doubly unmethylated PABP1 control peptide (left) or antigen-specific peptide (right).

Confocal immunofluorescent analysis of HeLa cells using Methyl-PAPBP1 (Arg 455/Arg460) (C60A10) Rabbit mAb (green). Actin filaments have been labled with DY-554 phalloidin (red). Blue Pseduocolor = DRAQ5™ (fluorescent DNA dye).

Background

Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.

Poly(A)-binding protein 1 (PABP1)与mRNA的3' poly(A)尾及eIF4F蛋白相联系(1,2)。 eIF4F是一个复合体,它的功能主要有mRNA 5'帽子结构 识别(eIF4E)、RNA解旋酶呈递到5'端区域(eIF4A)、mRNA与核糖体建立连接(eIF4G)、通过 eIF4G和poly(A) binding protein (PABP)相互作用使mRNA环化。而研究显示PABP1有多种功能,包括翻译起始、mRNA稳定和mRNA降解(3,4)。PABP的磷酸化能提高真核生物RNA结合,并且PABP1在细胞核与细胞质穿梭(5,6)。甲基化转移酶CARM1蛋白使PABP1蛋白在Arg455和Arg460位点发生甲基化(7,8);然而这个甲基化后的功能还没有确定。

  1. Sachs, A. B. et al. (1986) Cell 45, 827-835.
  2. Piron, M. et al. (1998) EMBO J. 17, 5811-5821.
  3. Caponigro, G. and Parker, R. (1995) Genes Dev. 9, 2421-2432.
  4. Sachs, A.B. and Davis, R.W. (1989) Cell 58, 857-867.
  5. Le, H. et al. (2000) J. Biol. Chem. 275, 17452-17462.
  6. Afonina, E. et al. (1998) J. Biol. Chem. 273, 13015-13021.
  7. Lee, J. and Bedford, M.T. (2002) EMBO Rep 3, 268-73.
  8. Yadav, N. et al. (2003) Proc Natl Acad Sci U S A 100, 6464-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications