Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-CrkII (Tyr221) Antibody #3491

No. Size Price
3491S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3491 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Hamster, Endogenous 42 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-CrkII (Tyr221) Antibody detects endogenous levels of CrkII only when phosphorylated at tyrosine 221. The antibody cross-reacts with Tyr207-phosphorylated CrkL but does not cross-react with other tyrosine-phosphorylated proteins. Phospho-CrkII (Tyr221) Antibody仅检测tyrosine 221位点磷酸化的内源性CrkII蛋白。该抗体与Tyr207位点磷酸化CrkL蛋白发生交叉反应,但是不能与其它tyrosine位点磷酸化蛋白质发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr221 of human CrkII. Antibodies are purified by protein A and peptide affinity chromatography. 通过人工合成人源CrkII蛋白Tyr221位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-CrkII (Tyr221) Antibody. 使用Phospho-CrkII (Tyr221) Antibody,免疫印迹(Western Blot)分析K562细胞系中Phospho-CrkII (Tyr221)蛋白水平,细胞分为未处理组和calf intestinal phosphatase (CIP)处理组。


CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3). CrkII蛋白是一个细胞内v-Crk的同系物,该蛋白属于含有SH2-SH3-SH3结构域的受体蛋白家族,上述结构域能传递来自酪氨酸激酶的信号(1)。Crk蛋白的主要功能是通过SH2-磷酸化-酪氨酸的相互作用招募在酪氨酸激酶附近的细胞质蛋白。因此,来自Crk蛋白的输出取决于SH3结合蛋白,这包括C3G和Sos鸟嘌呤核苷酸交换蛋白、Abl酪氨酸激酶、DOCK180和一些 STE20相关激酶。Crk结合蛋白的种类表明Crk蛋白的多种功能(2)。CrkII蛋白两个 SH3结构域通过一个54氨基酸连接区被分开,这在爪蟾属、小鸡和哺乳类CrkII蛋白中高度保守(3)。在这个区域Tyrosine 221位点通过Abl酪氨酸激酶(4)、IGF-I受体(5)和EGF受体(6)磷酸化。一旦Tyr221位点被磷酸化,CrkII蛋白在分子内折叠和SH2-pTyr 相互作用方面有一个改变,这会引起CrkII蛋白从酪氨酸激酶复合物上快速分离(3)。

  1. Zvara, A. et al. (2001) Oncogene 20, 951-961.
  2. Kiyokawa, E. et al. (1997) Crit. Rev. Oncog. 8, 329-342.
  3. Rosen, S. K. et al. (1995) Nature 374, 477-479.
  4. Amoui, M. and Miller, W.T. (2000) Cell. Signal. 12, 637-643.
  5. Koval, A. P. et al. (1998) Biochem. J. 330, 923-932.
  6. Hashimoto, Y. et al. (1998) J. Biol. Chem. 273, 17186-17191.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :