Cell Signaling Technology

Product Pathways - Metabolism

Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb #3482

LKB   PJS   renal-carcinoma-antigen-NY-REN-19   sc-271924   STK11  

No. Size Price
3482S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3482 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 54 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb detects endogenous levels of LKB1 when phosphorylated at Ser428.

Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb用于检测内源性在Ser428位磷酸化的LKB1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser428 of human LKB1 (Ser431 of mouse).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, untreated or treated with TPA #9905 and UV, using Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb (upper) and LKB1 (D60C5) Rabbit mAb #3047 (lower).

Wetern blot检测多种细胞提取物,细胞未处理或用TPA #9905 和UV处理,所用抗体为Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb兔单抗 (上)和 LKB1 (D60C5) Rabbit mAb 兔单抗#3047 (下)。


LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, 
including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). 
 Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

LKB1 (STK11)是一种丝氨酸/苏氨酸蛋白激酶,通过调控多种下游激酶信号通路来调控细胞结构,凋亡和能量平衡(1,2)。一个包括有LKB1,假定的激酶STRAD和MO25 脚手架蛋白和胞质蛋白复合体可以激活AMPK和AMPK相关激酶的活性(3)。AMPK是细胞能量平衡中的主要调控蛋白,控制多种下游的效应蛋白,在接受ATP浓度信号时发挥细胞生长和凋亡的调控作用(4)。在细胞内LKB1可以在多个位点被磷酸化,包括 Ser31/325/428 和 Thr189/336/363 (5)。LKB1的突变会引起Peutz-Jeghers综合征,该疾病是一种常染色体显性遗传疾病,主要特征是良性胃肠息肉和口、手、足上的黑色皮肤损伤(6)。LKB1其它的一些突变跟几种组织中弥散的肿瘤有关(7)。

Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 (8) as well as regulate apoptosis through PTEN signaling pathway suppression of Akt (9).


  1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
  2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
  3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
  4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
  5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
  6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
  7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.
  8. Xie, Z. et al. (2006) J Biol Chem 281, 6366-75.
  9. Song, P. et al. (2008) J Biol Chem 283, 12446-55.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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