Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb #3476

bek   fgf   FGFR   FGFR-1   fibroblast growth factor receptor   flg   KGFR  

No. Size Price
3476L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3476S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3476 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 120, 145 Mouse IgG2b

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb detects transfected levels of FGF receptors only when phosphorylated at tyrosines 653/654. The antibody cross-reacts slightly with activated PDGF and insulin/IGF-I receptors.

磷酸化FGF Receptor (Tyr653/654) (55H2) 鼠单抗仅在Tyr 653/654被磷酸化后才能检测到转染表达的FGFR蛋白的存在。本抗体能与活化的PDGF和胰岛素/IGF-I receptors有轻微的交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr653/654 of human FGF receptor-1. The corresponding sequence is identical in FGF receptor-2, -3 and -4.

单克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的FGFR1蛋白Tyr653/654附近的氨基酸序列合成的,与 FGFR-2, -3 and -4上对应的序列相同。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells overexpressing human FGF receptor-1, untreated or calf intestine phosphatase (CIP)-treated, using Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb. Overexpression of human FGF receptor-1 results in constitutive activation of the receptors (courtesy of Dr. Pamela Maher, Scripps Research Institute, California, personal communication). CIP treatment abolishes the reactivity of this antibody to FGF receptor-1. Western blot法检测高表达人FGFR1的COS细胞提取物:经过或未经calf intestinal phosphatase (CIP)处理。使用抗体为:Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb 。细胞高表达人FGFR-1蛋白后使受体组成型激活(courtesy of Dr. Pamela Maher, Scripps Research Institute, California, personal communication)。CIP 处理则能破坏本抗体对FGFR-1的反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-204 cells treated with or without bFGF, using Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb (upper) and FGF Receptor 1 (D8E4) XP® Rabbit mAb (lower).

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

成纤维细胞生长因子 (FGFs)通过细胞表面的酪氨酸激酶受体传递信号,对靶细胞产生促有丝分裂和血管生成的效应。已知FGF受体家族有四个成员:FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3和FGFR-4。每个受体都有一个胞外配体结合区、跨膜区和一个胞内激酶区(1)。在结合配体和二聚化之后,受体的特定的酪氨酸位点发生磷酸化(2)。在FGFR-1的羧基端有7个酪氨酸位点能被磷酸化:Tyr463, 583, 585, 653, 654, 730和 766。Tyr653 和Tyr654对激活的FGFR的催化活性十分重要,对信号传导也是必要的(3)。其它的磷酸化酪氨酸位点能够为下游信号分子,如Crk 和PLCγ,提供结合位点(4,5)。

  1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
  2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
  3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
  4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
  5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

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