Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-DRP1 (Ser616) Antibody #3455

DLP1   DNM1L   Dynamin-like protein 1   Dynamin-related protein 1  

No. Size Price
3455S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3455 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 78-82 Rabbit
IP 1:50
F 1:100
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-DRP1 (Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616.

Phospho-DRP1 (Ser616)抗体可以识别616位丝氨酸磷酸化的内源性DRP1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser616 of human DRP1. Antibodies are purified by protein A and peptide affinity chromatography.

多抗由合成肽段免疫动物产生,该肽段与人DRP1蛋白616位丝氨酸附近氨基酸对应。抗体由蛋白A和肽段亲和层析技术纯化得到。

IP

IP

Immunoprecipitation of Phospho-DRP1 (Ser616) from HeLa cell extracts, untreated or nocodazole-treated, using Phospho-DRP1 (Ser616) Antibody followed by western blot using the same antibody. Lanes 1 & 2 are 5% input.使用Phospho-DRP1 (Ser616)抗体对未处理或经过nocodazole处理的HeLa细胞中的Phospho-DRP1 (Ser616)进行免疫共沉淀实验。Phospho-DRP1 (Ser616)抗体也同样用于western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated for the indicated times, using Phospho-DRP1 (Ser616) Antibody.使用Phospho-DRP1 (Ser616)抗体对未处理或经过nocodazole处理一定时间的HeLa细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-DRP1 (Ser616) Antibody.使用Phospho-DRP1 (Ser616)抗体对未处理或经过Calyculin A #9902处理的HeLa细胞提取物进行western blot分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-DRP1 (Ser616) Antibody versus propidium iodide (DNA content).使用Phospho-DRP1 (Ser616)抗体和碘化丙啶(DNA content)对Jurkat细胞进行流式分析。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H1299 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-DRP1 (Ser616) Antibody (green). Actin filaments have been labeled with DY-554 phallodin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).未处理(左)或经过λ-phosphatase处理(右)的NCI-H1299细胞,使用Phospho-DRP1 (Ser616)抗体(绿色)进行激光共聚焦实验。肌动蛋白丝使用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® (fluorescent DNA dye).

Background

Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6).

Dynamin-related protein 1 (DRP1)是GTPases dynamin超家族的一员。该家族的成员具有多种功能包括囊泡分离,细胞器分裂,对抗病毒以及胞内示踪(1)。DRP1影响线粒体的形态,对哺乳动物细胞中线粒体和过氧化物酶病裂变有重要作用(2-5)。DRP1的酵母同源蛋白成簇排列在线粒体分裂位置的线粒体膜螺旋状结构处(6),这个结构似乎在哺乳动物细胞中也是保守的(3)。线粒体的分裂部分通过DRP1的616位丝氨酸被Cdk1/cyclin B磷酸化和637位丝氨酸被蛋白激酶A(PKA)磷酸化调控(6),线粒体的分裂对细胞凋亡、正常细胞生长和发育都是必须的。616位丝氨酸被磷酸化后,DRP1刺激有丝分裂时的线粒体分裂。相反的,DRP1的637位丝氨酸被磷酸化后分裂受限(6)。磷酸酶去磷酸化637位丝氨酸后,抑制被翻转(7)。除了磷酸化,DRP1的SUMO化也是线粒体分裂的促进因素(8)。分裂和融合之间的平衡是正常发挥线粒体功能所必需的。研究证明线粒体缺陷会导致多种神经功能障碍包括阿尔兹海默症,帕金森病已经亨廷顿症(6)。

  1. Praefcke, G.J. and McMahon, H.T. (2004) Nat Rev Mol Cell Biol 5, 133-47.
  2. Taguchi, N. et al. (2007) J Biol Chem 282, 11521-9.
  3. Smirnova, E. et al. (2001) Mol Biol Cell 12, 2245-56.
  4. Smirnova, E. et al. (1998) J Cell Biol 143, 351-8.
  5. Koch, A. et al. (2003) J Biol Chem 278, 8597-605.
  6. Knott, A.B. et al. (2008) Nat Rev Neurosci 9, 505-18.
  7. Cereghetti, G.M. et al. (2008) Proc Natl Acad Sci USA 105, 15803-8.
  8. Zunino, R. et al. (2007) J Cell Sci 120, 1178-88.

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