Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Doublecortin (Ser334) Antibody #3453

No. Size Price
3453S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3453 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 45 Rabbit
IP 1:50
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Phospho-Doublecortin (Ser334) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser334.

Phospho-Doublecortin (Ser334) Antibody 兔多抗识别内源性的Ser334磷酸化的doublecortin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho- or non-phosphopeptide and by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.

Western blot分析新生小鼠和胎大鼠大脑提取物,使用的抗体是Phospho-Doublecortin (Ser334) Antibody 兔多抗。抗体的磷酸化特异性由磷酸化或非磷酸化肽段阻断,在western转膜后对膜进行(+)或不进行(-)小牛肠碱性磷酸酶(CIP)处理。



Confocal immunofluorescent analysis of postnatal day 14 rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-Doublecortin (Ser334) Antibody (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析14日龄大鼠大脑,未经处理(左图)或经过λ磷酸酶处理(右图),使用Phospho-Doublecortin (Ser334) Antibody 兔多抗(绿色)和GFAP (GA5) Mouse mAb 鼠单抗#3670(红色)进行标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。


Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3).


  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
  3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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