Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Doublecortin (Ser334) Antibody #3453

No. Size Price
3453S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3453 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 45 Rabbit
IP 1:50
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Phospho-Doublecortin (Ser334) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser334.

Phospho-Doublecortin (Ser334) Antibody 兔多抗识别内源性的Ser334磷酸化的doublecortin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类doublecortin蛋白Ser334位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho- or non-phosphopeptide and by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.

Western blot分析新生小鼠和胎大鼠大脑提取物,使用的抗体是Phospho-Doublecortin (Ser334) Antibody 兔多抗。抗体的磷酸化特异性由磷酸化或非磷酸化肽段阻断,在western转膜后对膜进行(+)或不进行(-)小牛肠碱性磷酸酶(CIP)处理。

IF-F

IF-F

Confocal immunofluorescent analysis of postnatal day 14 rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-Doublecortin (Ser334) Antibody (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析14日龄大鼠大脑,未经处理(左图)或经过λ磷酸酶处理(右图),使用Phospho-Doublecortin (Ser334) Antibody 兔多抗(绿色)和GFAP (GA5) Mouse mAb 鼠单抗#3670(红色)进行标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Background

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3).

Doublecortin的突变可以导致无脑回畸形,一种伴有癫痫发作和智力迟滞的神经迁移障碍(1)。Doublecortin是一个微管相关的蛋白,可以稳定和束集微管蛋白。Doublecortin中的一个保守结构域介导其与微管的相互作用,有趣的是,大多数无义突变都发生在这个区域(2)。JNK、CDK5和PKA激酶可以磷酸化Doublecortin。JNK磷酸化Thr321、Thr331和Ser334;PKA磷酸化Ser47;CDK5磷酸化Ser297(3-5)。Ser297的磷酸化降低了Doublecortin与微管的结合。另外,Ser297突变可以导致迁移障碍(5)。Ser334磷酸化的Doublecortin在生长锥富集,影响神经节的生长和神经细胞的迁移(3)。

  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
  3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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