Cell Signaling Technology

Product Pathways - Development

Notch1 (C37C7) Rabbit mAb #3439

nicd   Notch   sc-6014  

No. Size Price
3439S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3439 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 120, 300 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

Notch1 (C37C7) Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~300 kDa) and the transmembrane/intracellular region NTM (~120 kDa).

Notch1 (C37C7)Rabbit mAb兔单抗识别内源性的总Notch1 蛋白。它识别Notch1 的全长蛋白(~ 300 kDa)和跨膜/胞内区域NTM(~120 kDa).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln2497 of human Notch1.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Notch1 (C37C7) Rabbit mAb.

使用Notch1 (C37C7) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM) for 3 days and then either harvested immediately (left panel) or washed and cultured in fresh media for 3 h (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 10 µl of Notch1 (C37C7) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

CUTLL1细胞在包含γ-分泌酶抑制剂(1 μM) 的培养基中培养三天后,直接收取细胞(左)或清洗后在新鲜培养基中培养3小时。使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 CUTLL1细胞与10μl的Notch1 (C37C7) Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human HES1 Promoter 引物,#7273 human HES4 promoter 引物,和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量相对于input染色质总量进行相对定量,Input中染色质量设定值为1。


Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.

Notch1是一个在细胞发育和命运决定过程中发挥功能的跨膜蛋白(1)。在notch1成熟过程中,notch1分子细胞外结构域会被一个成对碱性氨基酸蛋白酶样的转化酶剪切(2)。在与配体如Delta1结合或是细胞外钙离子耗竭后,notch1的羧基端被释放并随后在Gly1743 和 Val1744之间被切开(3,4)。结果导致细胞内处于活化状态的notch1片段转运至细胞核内并转录激活。

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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