Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438

No. Size Price
3438S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3438T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3438 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 58 to 68 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb detects PDK1 only when phosphorylated at Ser241.

Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb兔单抗能够检测Ser241位点磷酸化的内源性的PDK1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser241 of human PDK1.


Western Blotting

Western Blotting

Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower).

Western blot分析PC3 细胞提取物,未处理组和λ磷酸酶处理组, 所用抗体为Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 兔单抗(上), PDK1 Antibody #3062 (中) 和 Akt Antibody #9272 (下)。

Western Blotting

Western Blotting

Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

Western blot分析PC3、 HCT116 wild-type 和 HCT116 PDK1 -/- cells 细胞提取物,所用抗体为Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 兔单抗(上) 和 Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691 (下). (HCT116 wild-type和HCT116 PDK1 -/- 细胞 由Johns Hopkins University, Baltimore, MD的Dr. Bert Vogelstein友情提供)。


Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.Several serine sites (Ser25, Ser241, Ser393/396 and Ser410) are phosphorylated on PDK1 in unstimulated human embryo kidney 293 cells, as well as IGF-1 stimulated cells (4). Phosphorylation on the activation loop Ser241 by autophosphorylation is necessary for PDK1 activity (4).

磷酸肌醇依赖蛋白激酶1(PDK1)在多种信号传导通路中发挥核心作用(1,2),包括Akt和PKC同工酶p70 S6激酶和RSK的活化(3)。通过这些激酶,PDK1参与调控了广泛的生物学过程,包括细胞增殖,分化和凋亡。在未刺激的人类胚肾293细胞和IGF-1刺激的细胞中,PDK1的几个丝氨酸位点(Ser25, Ser241, Ser393/396 and Ser410)发生磷酸化(4)。在活化环Ser241位点的自磷酸化对于PDK1的活性是必须的(4)。

  1. Belham, C. et al. (1999) Curr. Biol. 9, R93-R96.
  2. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188.
  3. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448.
  4. Casamayor, A. et al. (1999) Biochem J 342 (Pt 2), 287-92.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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