Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-NuMA (Ser395) Antibody #3429

centrophilin   Nuclear mitotic apparatus protein 1   NuMA1   p240   SP-H Antigen   W1 antigen  

No. Size Price
3429S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3429 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 238 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-NuMA (Ser395) Antibody detects endogenous levels of NuMA protein only when phosphorylated at Ser395. Phospho-NuMA (Ser395) Antibody能够检测内源性丝氨酸(395位)磷酸化的NuMA蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser395 of human NuMA. Antibodies are purified using protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对NuMA蛋白丝氨酸(395位)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from M059K cells, untreated or UV-treated, using Phospho-NuMA (Ser395) Antibody. western blot检测未处理和紫外处理的M059K细胞提取物,使用的抗体为Phospho-NuMA (Ser395) Antibody。


The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4). 核有丝分裂器蛋白(NuMA)是一种参与有丝分裂纺锤体形成和维持的卷曲蛋白。NuMA在分裂间期的染色质组织过程起其重要作用,从而影响乳腺细胞分化(1,2)。凋亡过程中,NuMA的羧基末端清除可以放大细胞死亡信号通路。NuMA含有多个磷酸化位点,ATM/ATR依赖方式发生的丝氨酸395位磷酸化能够响应DNA损伤(3,4)。

Phospho-NuMA (Ser395) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser395 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information. Phospho-NuMA (Ser395) Antibody 作用位点是基于 PhosphoScan®和CST's LC-MS/MS 修饰位点发现平台,由Cell Signaling Technology (CST)公司鉴定的。采用ATM/ATR底物抗体能够发现395位丝氨酸的磷酸化,紫外处理能够诱导该磷酸化的发生。更多信息请访问CST修饰位点数据库(PhosphoSitePlus®)网址为www.phosphosite.org 。

  1. Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
  2. Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
  3. Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
  4. Matsuoka, S. et al. (2007) Science 316, 1160-6.
  5. Kotak, S. et al. (2013) EMBO J 32, 2517-29.
  6. Seldin, L. et al. (2013) Mol Biol Cell 24, 3651-62.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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