Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Stathmin (Ser38) Antibody #3426


No. Size Price
3426S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3426 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 19, 20 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-Stathmin (Ser38) Antibody detects endogenous levels of stathmin protein only when phosphorylated at Ser38.

Phospho-Stathmin (Ser38) Antibody检测仅在Ser38位点磷酸化的内源性stathmin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser38 of human stathmin. Antibodies are purified using protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated or treated with nocodazole for 16 hours at 100 ng/mL and unblocked (left), blocked with phospho-stathmin (Ser38) peptide (middle) or phospho-stathmin (Ser16) peptide (right), using Phospho-Stathmin (Ser38) Antibody.

使用Phospho-Stathmin (Ser38) Antibody,免疫印迹(Western Blot)分析HT-29细胞中Phospho-Stathmin蛋白水平。细胞分为untreated或100 ng/mL nocodazole-treated(16小时),抗体分别孵育unblocked (左图)、phospho-stathmin (Ser38) peptide (中图)或phospho-stathmin (Ser16) peptide (右图)。


Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8).

Stathmin蛋白是一个广泛表达的微管不稳定的磷蛋白(phosphoprotein),该蛋白在许多癌症中被上调。该蛋白的氨基端包含多个磷酸化位点,并且涉及促进tubulin纤维解聚作用。这些位点的磷酸化使该蛋白失活和稳定微管。在G2/M期,通过CaM kinases II and IV(1,2)使Ser16位点磷酸化增加,并涉及有丝分裂纺锤体打的调节(3,4)。Ser38是cdc2激酶的靶蛋白 (5),并且TNF诱导的细胞死亡引起活性氧中间产物,这导致stathmin蛋白的过度磷酸化(6)。Rac和cdc42蛋白的EGF受体激活也增加stathmin蛋白在Ser16和Ser38位点的磷酸化(7)。其它紧密相关的家族成员是神经元性表达,并且包括SCG10、SCLIP、RB3和它的剪切体RB3'、RB3''。Stathmin和SCG10蛋白已经证明在PC12细胞神经样发育中起着一定作用(8)。

  1. Marklund, U. et al. (1994) Eur J Biochem 225, 53-60.
  2. le Gouvello, S. et al. (1998) J Immunol 161, 1113-22.
  3. Mistry, S.J. and Atweh, G.F. (2001) J Biol Chem 276, 31209-15.
  4. Gavet, O. et al. (1998) J Cell Sci 111 ( Pt 22), 3333-46.
  5. Luo, X.N. et al. (1994) J Biol Chem 269, 10312-8.
  6. Vancompernolle, K. et al. (2000) J Biol Chem 275, 33876-82.
  7. Daub, H. et al. (2001) J Biol Chem 276, 1677-80.
  8. Di Paolo, G. et al. (1996) J Cell Biol 133, 1383-90.

Application References

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