Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PI3 Kinase p110 δ (D1Q7R) Rabbit mAb #34050

No. Size Price
34050S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
34050 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 110 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PI3 Kinase p110 δ (D1Q7R) Rabbit mAb recognizes endogenous levels of total PI3 Kinase Class III δ protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His481 of human PI3 Kinase Class III δ protein.

Western Blotting

Western Blotting

Western blot analysis of SW620 and HT-1080 cell extracts using PI3 Kinase p110 δ (D1Q7R) Rabbit mAb.

IP

IP

Immunoprecipitation of PI3 Kinase p110 δ from SW620 cells. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PI3 Kinase p110 delta (D1Q7R) Rabbit mAb. Western blot analysis was performed using PI3 Kinase p110 δ (D1Q7R) Rabbit mAb.

Background

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
  3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
  4. Stoyanov, B. et al. (1995) Science 269, 690-3.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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