Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-NMDAR1 (Ser897) Antibody #3385

amparnmdar   n-methyl-d-aspartate receptor   nmda receptor 1   nr1  

No. Size Price
3385S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3385 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 120 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-NMDAR1(Ser897) Antibody detects transfected NMDAR1 only when phosphorylated at serine 897.

Phospho-NMDAR1(Ser897) Antibody 兔多抗识别转染的Ser896磷酸化的NMDAR1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser897of human NMDAR1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类NMDAR1蛋白Ser897位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from parental (COS) or NMDAR1-transfected (COS/NMDAR1) cells, untreated or forskolin-treated (30 µM of for 20 minutes), using Phospho-NMDAR1 (Ser897) Antibody.

Western blot分析亲代(COS)或NMDAR1转染的(COS/NMDAR1)细胞,未处理或30 uM forskolin处理20分钟,使用的抗体是Phospho-NMDAR1 (Ser897) Antibody 兔多抗。

Background

N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).

N-甲基-D-天冬氨酸(NMDA)受体形成至少有一个NR1和一个NR2A-D亚基的异二聚体。其各种在脑内分布和功能特性不同的亚型是由NR1的选择性剪切和NR2的差异表达所形成。NR1亚基结合的共刺激激动剂甘氨酸,NR2结合的神经递质谷氨酸。NMDA受体激活或离子通道的开放,允许Na+和Ca2+离子进入细胞和K+流出细胞(1)。每个亚基有一个细胞质域,可直接通过蛋白激酶/磷酸酶进行修饰调控(2)。蛋白激酶C可以在Ser890/Ser896磷酸化NR1亚基(NMDAR1),PKA可以磷酸化NR1的Ser897(3)。NR1的PKC磷酸化降低其对钙调蛋白的亲和力,从而防止钙调蛋白对NMDA受体的抑制作用(4)。PKA磷酸化NR1可能可抵消钙调蛋白对受体的抑制作用(5)。NMDA受体介导长时程增强和慢速突触后兴奋,从而在学习,神经发育和神经可塑性中发挥中心作用(6)。

  1. Liu, X.B. et al. (2004) J Neurosci 24, 8885-95.
  2. Westphal, R.S. et al. (1999) Science 285, 93-6.
  3. Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66.
  4. Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10.
  5. Raman, I.M. et al. (1996) Neuron 16, 415-21.
  6. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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