Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-NMDAR1 (Ser890) Antibody #3381

amparnmdar   N-methyl-D-aspartate receptor   NMDA receptor 1   NR1  

No. Size Price
3381S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3381 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Transfected Only 120 Rabbit
IF-F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Phospho-NMDAR1 (Ser890) Antibody detects transfected NMDAR1 only when phosphorylated at serine 890.

Phospho-NMDAR1 (Ser890) Antibody 识别内源性的Ser890磷酸化的NMDAR1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 890 of human NMDAR1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from parental (COS) or NMDAR1-transfected (COS/NMDAR1) cells, untreated or TPA-treated (100 nM for 5 minutes), using Phospho-NMDAR1 (Ser890) Antibody.

Western blot分析亲代(COS)或NMDAR1转染的(COS/NMDAR1)细胞,未处理或100nM TPA处理5分钟,使用的抗体是Phospho-NMDAR1 (Ser890) Antibody 兔多抗。



Confocal microscopic image of mouse retina labeled with Phospho-NMDAR1 (Ser890) Antibody (red) and CREB (86B10) Mouse mAb #9104 (green). Blue pseudeocolor stain = DRAQ5® (fluorescent DNA dye).

共聚焦免疫荧光分析小鼠视网膜,使用Phospho-NMDAR1 (Ser890) Antibody 兔多抗(红色)和CREB (86B10) Mouse mAb 鼠单抗 #9104(绿色)进行标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。


N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).


  1. Liu, X.B. et al. (2004) J Neurosci 24, 8885-95.
  2. Westphal, R.S. et al. (1999) Science 285, 93-6.
  3. Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66.
  4. Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10.
  5. Raman, I.M. et al. (1996) Neuron 16, 415-21.
  6. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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