Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

PCAF (C14G9) Rabbit mAb #3378

acetylation   acetyltransferase   HAT   sc-8999  

No. Size Price
3378S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3378T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
3378 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 93 Rabbit IgG
IP 1:100
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Bovine, Horse,

Specificity / Sensitivity

PCAF (C14G9) Rabbit mAb detects endogenous levels of total PCAF protein. The antibody does not cross-react with the related GCN5L2 protein.

PCAF (C14G9) Rabbit mAb能够检测内源性PCAF总蛋白水平。该抗体不与相关的GCN5L2蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the human PCAF protein.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 µM) and either 20 μl of PCAF (C14G9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,来自处理了Forskolin #3828 (30 µM)的4 x 106 293细胞的交联染色质以及20 µl PCAF (C14G9) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用human ALS2 exon 1 primers、SimpleChIP® Human NR4A3 Promoter Primers #4829和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于总input chromatin的数量的信号,这相当于一。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PCAF (C14G9) Rabbit mAb.

使用PCAF (C14G9) Rabbit mAb,免疫印迹(Western blot)分析不同细胞中PCAF (C14G9)的蛋白水平。


p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B), is a transcriptional adaptor protein and histone acetyl-transferase (HAT) that functions as the catalytic subunit of the PCAF transcriptional co-activator complex (1). PCAF is 73% homologous to GCN5L2, another HAT protein found in similar complexes (1,2). Like GCN5L2, PCAF acetylates histone H3 on Lys14 and histone H4 on Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (3). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD), general transcription factors (TFIIEβ and TFIIF) and architectural DNA binding proteins (HMGA1 and HMG17) (4-10). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association.

p300/CBP-associated factor (PCAF)也称为lysine acetyl-transferase 2B (KAT2B),它是一个转录接头蛋白和组蛋白乙酰转移酶(HAT),其功能作为PCAF转录辅助激活复合物的催化亚单位(1)。PCAF是与GCN5L2蛋白有73%同源性,另外的HAT蛋白发现在类似复合物中(1,2)。像GCN5L2一样,PCAF使histone H3蛋白Lys14位点和histone H4蛋白Lys8位点乙酰化,它们通过调节染色质结构和招募额外的辅助激活因子从而有助于基因激活,而辅助激活因子包含乙酰化的赖氨酸结合bromo区域(3)。PCAF也使非组蛋白乙酰化包括转录激活因子(p53, E2F1, MyoD) 、一般转录因子(TFIIEβ and TFIIF)和结构性DNA结合蛋白(HMGA1和HMG17) (4-10)。这些蛋白的乙酰化可以调节它们的核定位、蛋白质稳定性、DNA结合和辅助激活因子的关联性。

  1. Nagy, Z. and Tora, L. (2007) Oncogene 26, 5341-57.
  2. Yang, X.J. et al. (1996) Nature 382, 319-24.
  3. Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92.
  4. Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92.
  5. Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
  6. Martínez-Balbás, M.A. et al. (2000) EMBO J 19, 662-71.
  7. Sartorelli, V. et al. (1999) Mol Cell 4, 725-34.
  8. Imhof, A. et al. (1997) Curr Biol 7, 689-92.
  9. Munshi, N. et al. (1998) Mol Cell 2, 457-67.
  10. Herrera, J.E. et al. (1999) Mol Cell Biol 19, 3466-73.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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