Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

UTX (D3Q1I) Rabbit mAb #33510

de-methylase   demethylase   KDM6A  

No. Size Price
33510S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
33510 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 180 Rabbit IgG
IP 1:50
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

UTX (D3Q1I) Rabbit mAb recognizes endogenous levels of total UTX protein. This antibody does not cross-react with UTY protein. This antibody also cross-reacts with unidentified proteins of 60 kDa and 70 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Ala490 of human UTX protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UTX (D3Q1I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, THP-1 cells are negative for UTX.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using UTX (D3Q1I) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded colon carcinoma using UTX (D3Q1I) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 293 (left) and THP-1 (right) cell pellets using UTX (D3Q1I) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using UTX (D3Q1I) Rabbit mAb.

Background

The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The three members of the UTX/UTY family include the ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX), the ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (UTY) and JmjC domain-containing protein 3 (JMJD3) (3). This family of proteins has been shown to demethylate both di- and tri-methyl histone H3 Lys 27 (4-8). The UTX gene escapes X inactivation in females and is ubiquitously expressed (9). UTX functions to regulate HOX gene expression during development (4-6). JMJD3 functions to regulate gene expression in macrophages responding to various inflammatory stimuli and has been shown to be upregulated in prostate cancer (7,8). Both UTX and JMJD3 interact with mixed-lineage leukemia (MLL) complexes 2 and 3, both of which have been shown to methylate histone H3 at Lys4 (6,7). The UTY gene is expressed in most tissues in the male mouse (10).

  1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
  2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
  3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
  4. Agger, K. et al. (2007) Nature 449, 731-4.
  5. Lan, F. et al. (2007) Nature 449, 689-94.
  6. Lee, M.G. et al. (2007) Science 318, 447-50.
  7. De Santa, F. et al. (2007) Cell 130, 1083-94.
  8. Xiang, Y. et al. (2007) Cell Res 17, 850-7.
  9. Greenfield, A. et al. (1998) Hum Mol Genet 7, 737-42.
  10. Greenfield, A. et al. (1996) Nat Genet 14, 474-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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