Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348

NPM-ALK  

No. Size Price
3348S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3348 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 (NPM-ALK) 220 (ALK) Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-ALK (Tyr1586) (3B4) Rabbit mAb detects ALK only when phosphorylated at Tyr1586 (equivalent to Tyr646 of NPM-ALK). This antibody may cross-react with other activated protein tyrosine kinases including EGFR. 磷酸化的ALK(Tyr1586)(3B4)的兔源单克隆抗体(3B4)仅在Tyr1586(对应NPM-ALK上Tyr646)被磷酸化后才能检测到ALK的存在。本抗体与其它激活的酪氨酸激酶(如EGFR)有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1586 of human ALK. 多克隆抗体由合成磷酸化多肽免疫动物而得,该多肽设计在人ALK Tyr1586周围

Western Blotting

Western Blotting

Western blot analysis of Sup-M2 cell lysate, using Phospho-ALK (Tyr1586) (3B4) Rabbit mAb (A and B), and ALK Antibody (C and D).The phospho-specificity of this antibody was characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B and D) after Western transfer. 用抗磷酸化的ALK (Tyr1586)的兔源单克隆抗体(3B4)(A, B)或抗ALK的抗体(C, D)对Sup-M2细胞提取物进行蛋白质印记检测。转膜后用小牛肠碱性磷酸酶(CIP)对膜进行处理(B, D),可以检测磷酸化抗体的特异性。

Background

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and EML4 has been described from a non-small cell lung cancer cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6,7).

间变性淋巴瘤激酶(ALK)是多效生长因子(PTN)的酪氨酸激酶受体,它是大脑胚胎发育过程中的生长因子(1-3)。 在表达ALK的细胞中,PTN诱导ALK及其下游效应因子IRS-1, Shc, PLCγ, 和PI3K激酶发生磷酸化(1)。ALK最初是从易位突变产生的NPM-ALK融合蛋白而被发现的(4)。NPM-ALK融合蛋白是一个组成性活化,有原癌基因活性的间变性淋巴瘤相关酪氨酸激酶(4)。 由NPM-ALK介导的PLCγ的活化可能对有丝分裂活性起到关键作用,并且可能对间变性淋巴瘤的发病过程很重要(5)。另一个完全不同的ALK原癌融合蛋白在非小细胞肺癌细胞株中被报道,该蛋白融合了ALK与EML4,其相应融合转录产物在一些肺腺癌中也有表达。在EML4-ALK融合蛋白中,微管相关蛋白EML4的短氨基末端区域和ALK的激酶活性区域融合在一起(6,7)。

  1. Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.
  2. Iwahara, T. et al. (1997) Oncogene 14, 439-49.
  3. Morris, S.W. et al. (1997) Oncogene 14, 2175-88.
  4. Morris, S.W. et al. (1994) Science 263, 1281-4.
  5. Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.
  6. Rikova, K. et al. (2007) Cell 131, 1190-203.
  7. Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.
  8. Soda, M. et al. (2007) Nature 448, 561-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications