Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Dab1 (Tyr220) Antibody #3327

No. Size Price
3327S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3327 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 80, 110 GFP-Dab1 fusion. Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Dab1 (Tyr220) Antibody detects transfected levels of Dab1 protein only when phosphorylated at tyrosine 220.

Phospho-Dab1 (Tyr220) Antibody 兔多抗识别转染的Tyr220磷酸化的Dab1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr220 of human Dab1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of cell lysates from COS cells co-transfected with GFP-Dab1 and Fyn expression constructs, untreated and treated with calf intestinal phosphatase (CIP), using Phospho-Dab1 (Tyr220) Antibody.

Western blot分析共转染GFP-Dab1和Fyn表达载体的COS细胞提取物,未处理或用小牛肠碱性磷酸酶(CIP)处理,使用的抗体是Phospho-Dab1 (yr220) Antibody 兔多抗。


The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons (1,2). Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2 (3). It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 (4).

Reelin信号转导通路在神经发育中起着关键作用。Reelin是一种分泌糖蛋白,可以和神经元表面的极低密度脂蛋白受体、ApoER2或α3β1整联蛋白结合(1,2)。这些受体激活诱导胞内接头蛋白Disabled 1 (Dab1)的酪氨酸磷酸化。普遍认为,Src家族酪氨酸激酶对Dab1酪氨酸磷酸化是Reelin信号下游事件中最关键的。其氨基酸末端的磷酸酪氨酸连接(PTB)域使Dab1识别和连接到受体胞质段尾部的保守序列模体上。另外,PTB包含了一个能结合磷酸肌醇的Pleckstrin同源结合子域。Dab1 PTB域内磷酸肌醇结合区域是膜定位和独立于VLDLR和ApoER2时基础酪氨酸磷酸化所必需的。研究显示Src, CrkII, CrkL和Dock1与络氨酸磷酸化的Dab有联系。CrkII-Dab1相互作用需要Dab1在220或232处进行络氨酸磷酸化。

  1. Huang, Y. et al. (2005) Biochem. Biophys. Res. Commun. 331, 1460-1468.
  2. Luque, J.M. (2004) Brain Res. Dev. Brain Res. 152, 269-271.
  3. Morimura, T. et al. (2005) J. Biol. Chem. 280, 16901-16908.
  4. Chen, K. et al. (2004) J. Cell. Sci. 117, 4527-4536.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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