Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Ras-GRF1 (Ser916) Antibody #3321


No. Size Price
3321S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3321 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Transfected Only 155 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Ras-GRF1 (Ser916) Antibody detects transfected levels of Ras-GRF1 only when phosphorylated at serine 916. This antibody does not cross-react with phosphorylated Ras-GRF2.

Phospho-Ras-GRF1 (Ser916) Antibody 兔多抗识别转染水平的Ser916磷酸化的Ras-GRF1蛋白。此抗体不与磷酸化的Ras-GRF2蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 916 of mouse Ras-GRF1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (lane 1) or transfected with either wild-type (lanes 2 and 3) or Ala916 mutant (lanes 4 and 5) Ras-GRF1, using Phospho-Ras-GRF1 (Ser916) Antibody (upper) or Ras-GRF1 Antibody #3322 (lower). Transfected COS cells were untreated or treated forskolin as indicated.(Triple HA-tagged Ras-GRF1 expressing plasmids provided by Dr. R. Mattingly, Dept. of Pharmacology, Wayne State University, Michigan.)

Western blot分析COS细胞,未转染( Lane 1)、转染野生型(Lane 2、3)或ALA916突变型(Lane 4、5)的Ras-GRF1,使用的抗体是Phospho-Ras-GRF1 (Ser916) Antibody 兔多抗 (上图)或 Ras-GRF1 Antibody 兔多抗(下图)。转染的COS细胞未处理或经Forskolin处理。(Triple HA-tagged Ras-GRF1表达质粒由Dr. R. Mattingly, Dept. of Pharmacology, Wayne State University, Michigan提供.)


Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2). Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2). Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity (3). Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity (4). It has been reported that Ras-GRF1 also shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5).


  1. Mattingly, R.R. and Macara, I.G. (1996) Nature 382, 268-72.
  2. Farnsworth, C.L. et al. (1995) Nature 376, 524-7.
  3. Baouz, S. et al. (2001) J. Biol. Chem. 276, 1742-1749.
  4. Mattingly, R.R. (1999) J Biol Chem 274, 37379-84.
  5. Kiyono, M. et al. (2000) J. Biol. Chem. 275, 29788-29793.

Application References

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