Cell Signaling Technology

Product Pathways - Metabolism

ZIP7/SLC39A7 (D1O3A) Rabbit mAb #33176

No. Size Price
33176S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
33176 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 50 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

ZIP7/SLC39A7 (D1O3A) Rabbit mAb recognizes endogenous levels of total ZIP7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu201 of human ZIP7 protein.

IP

IP

Immunoprecipitation of ZIP7 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ZIP7/SLC39A7 (D1O3A) Rabbit mAb. Western blot analysis was performed using ZIP7/SLC39A7 (D1O3A) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and 293 cells using ZIP7/SLC39A7 (D1O3A) Rabbit mAb.

Background

The solute carrier family 39 (zinc transporter) member 7 (SLC39A7, ZIP7) is an ER and Golgi membrane protein that regulates cellular zinc homeostasis by controlling release of zinc from these organelles to the cytoplasm (1,2). Zinc release mediated by ZIP7 results in activation of protein kinases that are involved in cell proliferation and migration (3,4). The protein kinase CK2 phosphorylates and activates ZIP7 in response to extracellular signals, such as growth factor stimulation (4,5). Increased expression of ZIP7 is observed in breast cancer tissues (6). Research studies indicate that ZIP7 is responsible for activation of multiple tyrosine kinases in aggressive, tamoxifen-resistant breast cancer (7,8).

  1. Taylor, K.M. et al. (2004) Biochem J 377, 131-9.
  2. Huang, L. et al. (2005) J Biol Chem 280, 15456-63.
  3. Hogstrand, C. et al. (2009) Trends Mol Med 15, 101-11.
  4. Taylor, K.M. et al. (2012) Cell Cycle 11, 1863-4.
  5. Taylor, K.M. et al. (2012) Sci Signal 5, ra11.
  6. Taylor, K.M. (2008) Biochem Soc Trans 36, 1247-51.
  7. Taylor, K.M. et al. (2007) Mol Med 13, 396-406.
  8. Taylor, K.M. et al. (2008) Endocrinology 149, 4912-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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