Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316

mapk   mapkap   mapkap 2   mapkap-2   mapkap2   mapkapk   mapkapk 2   mapkapk-2   mapkapk2   mk-2   mk2  

No. Size Price
3316S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3316T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3316 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 49 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr222.

Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb兔单抗能检测内源性Thr222位点磷酸化的MAPKAPK-2蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr222 of human MAPKAPK-2.

该单克隆抗体是采用合成的与人源MAPKAPK-2蛋白Thr222位点周围残基相一致的磷酸化肽段免疫动物后获得的。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper) or MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物:未经处理的(-) 和紫外照射、TPA处理的(+)NIH/3T3细胞,使用的抗体是Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (上图)和 MAPKAPK-2 Antibody #3042 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物:未经处理的(-) 和紫外照射、TPA处理的(+)HeLa细胞,使用的抗体是Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (上图)和 MAPKAPK-2 Antibody #3042 (下图)。

Background

In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

在细胞因子、压力和化学因子的刺激下,丝裂原活化蛋白激酶激活的蛋白激酶2(MAPKAPK-2)能够迅速被磷酸化并被激活。研究显示MAPKAPK-2是p38 MAPK的直接靶点(1)。在体内,在压力应激下MAPKAPK-2的多个氨基酸都能被磷酸化。但是,在体外激酶实验中,仅有4个位点(Thr25, Thr222, Ser272, 和 Thr334)能够被p38 MAPK磷酸化(2)。Thr222, Ser272和Thr334的磷酸化对于MAPKAPK-2的激酶活性是必须的(2)。Thr25可以在体外被p42 MAPK磷酸化,但是该磷酸化对于MAPKAPK-2的激活不是必须的(2)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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