Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313

sc-12912  

No. Size Price
3313L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3313S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3313T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3313 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Bovine, Endogenous 19 Rabbit IgG
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at serine 3.

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb仅识别serine 3磷酸化的内源性cofilin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin.

通过人工合成人源cofilin蛋白Ser3邻近氨基酸序列一致的肽段,免疫动物从而制备出单克隆抗体。

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells either untreated (left) or λ phosphatase-treated (right), using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (绿色)标记,共聚焦免疫荧光分析MCF7细胞,细胞分为untreated组 (左图)和λ phosphatase-treated组(右图)。蓝色= DRAQ5® #4084(DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb. 使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb,免疫印迹(Western Blot)分析NIH/3T3细胞系中Phospho-Cofilin (Ser3)蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb. 使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb,免疫印迹(Western Blot)分析NIH/3T3细胞系中Phospho-Cofilin (Ser3)蛋白水平。

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

Cofilin和actin-解聚因子(ADF)都属于一组保守的小actin-结合蛋白家族,在胞质分裂,内吞,胚胎发育,压力应激和组织再生过程中发挥了关键作用(1)。在刺激因素作用下,cofilin作为提前存在的细丝促进actin肌动蛋白丝的再生(2)。Cofilin的第3位丝氨酸被LIMK或TESK磷酸化后会抑制cofilin的这种作用(3-5)。第3位丝氨酸磷酸化也会调控cofilin从细胞核转位到细胞质中(6)。

  1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
  2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
  3. Arber, S. et al. (1998) Nature 393, 805-9.
  4. Yang, N. et al. (1998) Nature 393, 809-12.
  5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
  6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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