Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Cofilin (Ser3) Antibody #3311

sc-12912  

No. Size Price
3311L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3311S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3311 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey,Bovine, Endogenous 19 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Pig,

Specificity / Sensitivity

Phospho-Cofilin (Ser3) Antibody detects endogenous levels of cofilin only when phosphorylated at serine 3. The antibody may cross-react with phosphorylated cofilin 2, the muscle isoform.

Phospho-Cofilin (Ser3) 抗体仅识别serine 3磷酸化的内源性cofilin总蛋白。该抗体可能与磷酸化的cofilin 2(muscle isoform)发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography.

通过合成与人源cofilin蛋白Ser3邻近氨基酸残基序列一致的肽段,免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or H2O2-treated, using Phospho-Cofilin (Ser3) Antibody (A and B) or Cofilin Antibody #3312 (C and D). Membranes (B and D) were treated with alkaline phosphatase (CIP) to demonstrate the phospho-specificity the antibody. 使用Phospho-Cofilin (Ser3) Antibody (A和B)或Cofilin Antibody #3312 (C和D),免疫印迹(Western Blot)分析HeLa细胞系,细胞分为untreated组或H2O2-treated组。alkaline phosphatase (CIP)处理膜(B和D)从而证明磷酸化特异性抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from asynchronous or mitotic C6, CHO and COS cells, using Phospho-Cofilin (Ser3) Antibody (upper) or Cofilin Antibody #3312 (lower). 使用Phospho-Cofilin (Ser3) Antibody (上图)或Cofilin Antibody #3312 (下图),免疫印迹(Western Blot)分析不同时期或有丝分裂期的C6、CHO和COS细胞系中Phospho-Cofilin (Ser3)和Cofilin蛋白水平。

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

Cofilin和actin-解聚因子(ADF)都属于一组保守的小actin-结合蛋白家族,在胞质分裂,内吞,胚胎发育,压力应激和组织再生过程中发挥了关键作用(1)。在刺激因素作用下,cofilin作为提前存在的细丝促进actin肌动蛋白丝的再生(2)。Cofilin的第3位丝氨酸被LIMK或TESK磷酸化后会抑制cofilin的这种作用(3-5)。第3位丝氨酸磷酸化也会调控cofilin从细胞核转位到细胞质中(6)。

  1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
  2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
  3. Arber, S. et al. (1998) Nature 393, 805-9.
  4. Yang, N. et al. (1998) Nature 393, 809-12.
  5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
  6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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