Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Pyk2 (Tyr402) Antibody #3291

CADTK   CAKbeta   RAFTK  

No. Size Price
3291S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3291 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 116 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-Pyk2 (Tyr402) Antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402. This antibody may cross-react with other phospho-tyrosine containing proteins.

Phospho-Pyk2 (Tyr402) Antibody兔多抗能检测内源性Tyr402位点磷酸化的Pyk2蛋白水平。该抗体可能与其他含有磷酸化酪氨酸的蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr402 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源 Pyk2蛋白Tyr402位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Immunoprecipitation of Pyk2 from Ramos cells, untreated, H2O2-treated, or Pervanadate-treated, using Pyk2 Antibody #3292, followed by Western blot with Phospho-Pyk2 (Tyr402) Antibody (upper), or Pyk2 Antibody #3292 (lower).免疫沉淀方法检测细胞中的Pyk2:未经处理和H2O2、过钒酸钠处理的Ramos细胞。使用的抗体是Pyk2 Antibody #3292。Western blot方法中使用的抗体是Phospho-Pyk2 (Tyr402) Antibody (上图)和Pyk2 Antibody #3292 (下图)。

Background

Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).

蛋白酪氨酸激酶Pyk2, 也被称为CAKβ, RAFTK和CADTK,是一种非受体酪氨酸激酶,在结构上与粘着斑激酶(FAK)相关(1-4)。Pyk2主要表达于来源于造血细胞系的细胞中和中枢神经系统中。Pyk2是G蛋白偶联受体和MAPK信号转导途径的一个信号转导调节子。它在细胞伸展和迁移过程中起着重要的作用(5-7)。

Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).

TCR的结合能够使Pyk2的酪氨酸磷酸化,从而被激活(8,9)。Pyk2蛋白Tyr402位点的磷酸化是Pyk2蛋白中其他酪氨酸的磷酸化所必须的,并且在T细胞活化过程中为Fyn SH2提供结合位点(10)。

  1. Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
  2. Lev, S. et al. (1995) Nature 376, 737-745.
  3. Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
  4. Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
  5. Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
  6. Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
  7. Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
  8. Berg, N.N. and Ostergaard, H.L. (1997) J. Immunol. 159, 1753-1757.
  9. Ganju, R. K. et al. (1997) J. Exp. Med. 185, 1055-1063.
  10. Katagiri, T. et al. (2000) J. Biol. Chem. 275, 19645-19652.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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