Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

ROS1 (D4D6®) Rabbit mAb #3287

No. Size Price
3287S 100 µl ( 10 western blots ) ¥7,500.00 现货查询 购买询价
3287T 20 µl ( 2 western blots ) ¥2,000.00 现货查询 购买询价
3287 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 258, 110, 50-80 Rabbit IgG
IP 1:50
IHC-P 1:250
F 1:100
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

ROS1 (D4D6) Rabbit mAb recognizes endogenous levels of total ROS1 protein. This antibody does not cross-react with other related proteins when analyzed by western blot. Please note that staining may be observed in ROS1 rearranged lung carcinomas, macrophages/giant cells, reactive type II pneumocyte hyperplasia, and the epithelium in areas of bronchiolar metaplasia. Staining of unknown specificity has been observed in cholangiocarcinoma, hepatocellular carcinoma, and kidney tissues.

ROS1 (D4D6) 兔单抗能够识别内源性水平的总ROS1蛋白。当用western blot进行分析时,该抗体不与其他相关蛋白发生交叉反应。请注意,染色可能在ROS1重排的肺癌,巨噬细胞/巨细胞,反应性Ⅱ型肺细胞增生,上皮细支气管上皮化生区域可见。在胆管癌,肝癌和肾脏中已观察到未知特异性的染色。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a protein corresponding to residues in the carboxy terminal domain of the human ROS1 protein.该


Western Blotting

Western Blotting

Western blot analysis of extracts from HCC78 (SLC34A2-ROS1), U-118 MG (FIG-ROS1), and HeLa (ROS1 negative) cells using ROS1 (D4D6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Note: HCC78 cells express the 85, 70, and 59 kDa forms of the SLC34A2-ROS1 fusion protein (7).Western blot方法检测HCC78 细胞(SLC34A2-ROS1), U-118 MG细胞 (FIG-ROS1)和 HeLa细胞(ROS1阴性)提取物,使用的抗体为ROS1 (D4D6) Rabbit mAb (上图)或β-Actin (D6A8) Rabbit mAb #8457 (下图)。注意:HCC78细胞表达85, 70和59 kDa的SLC34A2-ROS1融合蛋白压型(7)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ROS1 (D4D6) Rabbit mAb. Note: Staining is of FIG-ROS1 fusion (6).免疫组织化学方法检测石蜡包埋的人肺癌组织,使用的抗体为ROS1 (D4D6) Rabbit mAb。注意:染色的是FIG-ROS1融合蛋白(6)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinomas showing distinct localization using ROS1 (D4D6) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织,显示出明显的位置,使用的抗体为ROS1 (D4D6) Rabbit mAb。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (blue) and HCC78 (green) cells using ROS1 (D4D6) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.流式细胞术方法检测HeLa细胞(蓝色)和HCC78细胞(绿色),使用的抗体为ROS1 (D4D6) Rabbit mAb.使用的二抗为Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC78 xenograft using ROS1 (D4D6) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC78细胞移植物,使用的抗体为ROS1 (D4D6) Rabbit mAb.



Confocal immunofluorescent analysis of HCC78 (left) or HeLa (right) cells using ROS1 (D4D6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测HCC78细胞(左图)或HeLa细胞(右图),使用的抗体为ROS1 (D4D6) Rabbit mAb (绿色)。肌动蛋白丝用DY-554鬼笔环肽标记(红色)。蓝色伪彩色为DNA荧光染料,产品信息为DRAQ5® #4084。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing wildtype ROS1 (hROS1; +), using ROS1 (D4D6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测293T细胞提取物,细胞不转染(-)或用表达野生型ROS1(hROS1; +)的构建体进行转染,使用的抗体为ROS1 (D4D6) Rabbit mAb(上图)或β-Actin (D6A8) Rabbit mAb #8457 (下图)。



Immunoprecipitation of ROS1 from HCC78 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ROS1 (D4D6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ROS1 (D4D6) Rabbit mAb, followed by Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076.免疫沉淀方法检测HCC78细胞提取物中的ROS1,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或ROS1 (D4D6) Rabbit mAb (泳道3)。泳道1是加入10%蛋白。进行Western blot分析使用的抗体为ROS1 (D4D6) Rabbit mAb,随后用Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678和Anti-mouse IgG, HRP-linked Antibody #7076.


ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus (1). ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, a transmembrane domain, and an intracellular kinase domain. While the function of ROS1 is undefined, it has been shown to play an important role in differentiation of epididymal epithelium (2). The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma (3), and subsequent studies have found this fusion in cholangiocarcinoma (4), ovarian cancer (5) and non-small cell lung cancer (NSCLC) (6). Investigators have found additional oncogenic ROS1 fusion proteins in NSCLC (at a frequency of ~1.6%), where the ROS1 kinase domain is fused to the amino-terminal region of a number of different proteins, including CD74 and SLC34A2 (6-8). ROS1 fusion proteins activate the SHP-2 phosphatase, PI3K/Akt/mTOR, Erk, and Stat3 pathways (3,4,9).

ROS1是胰岛素受体家族的一个孤儿受体酪氨酸激酶,被初步确定为来自UR2肉瘤病毒的v-ros的同系物(1)。ROS1由一个大的胞外结构域,一个跨膜结构域和一个细胞内激酶结构域组成,其中胞外结构域由6个纤连蛋白重复序列组成。虽然ROS1的功能尚未定义,但已证明它在附睾上皮细胞分化中发挥重要的作用(2)。通过在胶质母细胞瘤中研究,初步确定第一个ROS1的致癌融合, FIG-ROS1(3),在随后的研究中发现这种融合在胆管癌(4),卵巢癌(5)和非小细胞肺癌(NSCLC)(6)中都存在。在非小细胞肺癌(1.6%的发生频率)中,研究者已经发现额外的致癌ROS1融合蛋白,其中ROS1的激酶结构域与若干种不同蛋白的氨基末端区域融合,这些蛋白包括CD74和SLC34A2(6-8)。ROS1融合蛋白激活SHP-2磷酸酶,PI3K/Akt/mTOR,ERK和STAT3通路(3,4,9)。

  1. Matsushime, H. et al. (1986) Mol Cell Biol 6, 3000-4.
  2. Yeung, C.H. et al. (1999) Biol Reprod 61, 1062-9.
  3. Charest, A. et al. (2003) Genes Chromosomes Cancer 37, 58-71.
  4. Gu, T.L. et al. (2011) PLoS One 6, e15640.
  5. Birch, A.H. et al. (2011) PLoS One 6, e28250.
  6. Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57.
  7. Rikova, K. et al. (2007) Cell 131, 1190-203.
  8. Stumpfova, M. and Jänne, P.A. (2012) Clin Cancer Res 18, 4222-4.
  9. Jun, H.J. et al. (2012) Cancer Res 72, 3764-74.

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