Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FAK (Tyr576/577) Antibody #3281

FAK   Focal adhesion kinase  

No. Size Price
3281S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3281T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3281 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 125 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus,

Specificity / Sensitivity

Phospho-FAK (Tyr576/577) Antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 576/577.

磷酸化FAK(酪氨酸576/577)抗体仅仅在酪氨酸576/577磷酸化时检测内源性FAK。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr576/577 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体由合成的磷酸化肽段免疫动物产生,该肽段与人FAK的酪氨酸576/577附近残基对应。抗体由蛋白A和肽段亲和层析纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody (upper) or FAK antibody (lower).未处理或经过anti-CD3抗体(1 µg/ml 10分钟)处理的Jurkat 细胞提取物使用 Phospho-FAK (Tyr576/577) 抗体(上)或FAK抗体(下)进行western blot分析。

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

黏着斑激酶(FAK)是一类广泛表达在细胞质的蛋白酪氨酸激酶,涉及整合素调节的信号转导,它起着重要的作用,在控制包括细胞扩散迁移和存活的一系列生物过程(1)。整合素成簇激活FAK,导致酪氨酸397的自磷酸化,这是一个Src家族激酶PI3K和PLCγ的结合位点(2-5). Src家族激酶的招募导致Tyr407, Tyr576和 Tyr577在催化结构域的磷酸化,以及Tyr871和Tyr925在FAK羧基末端区域的磷酸化(6,7)。

Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6).

酪氨酸576和酪氨酸577处于激酶结构域的激活环,这些残基的突变减弱FAK的催化活性(6)。

  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
  2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
  3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
  4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
  5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
  6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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