Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270

cjun  

No. Size Price
3270S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
3270T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
3270 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Pig, Endogenous 48 Rabbit IgG
IP 1:50
IHC-P 1:200
F 1:200
IF-IC 1:800
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb detects endogenous levels of c-Jun only when phosphorylated at Ser73.

Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb兔单抗能够检测内源性Ser73位点磷酸化的c-Jun蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun.

该单克隆抗体是采用合成的与人源c-Jun蛋白Ser73位点周围残基相一致的合成肽段免疫动物后产生的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or UV treated (green), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb.Flow cytometric方法检测细胞提取物:未经处理(蓝色)和紫外照射的(绿色)HeLa细胞,使用的抗体是Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测:未经处理的(左侧)或茴香霉素处理的(右侧)HeLa 细胞,使用的抗体是 Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (绿色),肌动蛋白纤维素已被 DY-554鬼笔环肽(红色)标记。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 or C6 cells, untreated or UV-treated, using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).Western blot方法检测细胞提取物:未经处理的和紫外照射的NIH/3T3、C6细胞。使用的抗体是Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (上图)和c-Jun (60A8) Rabbit mAb #9165 (下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人乳腺癌组织,左侧为对照组,右侧为lambda 磷酸酶处理的。使用的抗体为Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织,使用的抗体为Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of parafin-embedded human colon carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser73) Blocking Peptide (right).免疫组织化学方法检测石蜡包埋的人结肠癌组织,使用的抗体为Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (hβ-NGF) #5221 (50 ng/ml) for 2h and either 10 μl of Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® using Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

c-Jun是Jun家族的一个成员,Jun家族成员包括c-Jun, JunB, 和 JunD。c-Jun是转录因子激活蛋白-1(AP-1)的一个组分。AP-1是由Fos和Jun形成的二聚体以及 ATF家族成员组成的,能够结合并激活TRE/AP-1元件的转录(1)。包括生长因子,细胞因子和压力在内的细胞外信号能够激活AP-1依赖的转录过程。C-Jun的转录活性是通过SAPK/JNK对其中的Ser63和Ser73的磷酸化来调控的(2)。小鼠敲除实验表明c-Jun对于胚胎形成是至关重要的(3),进一步的研究发现c-Jun在多种组织和发育过程中发挥作用,包括轴突再生(4),肝脏再生(5)和T细胞发育(6)。AP-1所调控的基因发挥了多种生物学功能,包括细胞增殖、分化、凋亡、转化,侵润和转移,这依赖于细胞种类和具体情况(7-9)。其他的AP-1所调控的靶基因还能够调节细胞存亡,以及低氧和血管生成(8,10)。研究提示c-Jun可以作为癌症、血管重构、急性炎症反应和风湿性关节炎的重要治疗靶点(11,12)。

  1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
  2. Davis, R.J. (2000) Cell 103, 239-52.
  3. Hilberg, F. et al. (1993) Nature 365, 179-81.
  4. Raivich, G. et al. (2004) Neuron 43, 57-67.
  5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
  6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
  7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
  9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
  10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
  11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
  12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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