Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb #3233

GRB2-associated binder 1   GRB2-associated-binding protein 1   Growth factor receptor bound protein 2-associated protein 1  

No. Size Price
3233S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3233T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3233 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 110 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb detects endogenous levels of Gab1 only when phosphorylated at Tyr627. The antibody may cross-react with phosphorylated Gab2 or Gab3, or with activated receptor tyrosine kinases (RTKs).

Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb兔单抗能够检测内源性的酪氨酸(627位点)磷酸化的Gab1蛋白。抗体也可以与磷酸化的Gab2及磷酸化的Gab3或者活化的受体酪氨酸激酶(RTKs)发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1.


Western Blotting

Western Blotting

Western blot analysis of cell extracts from T47D cells, untreated or stimulated with heregulin, using Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb (upper) or Gab1 Antibody #3232 (lower). Western blot 检测分析T47D细胞提取物,未处理的和heregulin刺激的;使用的抗体是 Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb(上图)和Gab1 Antibody #3232 (下图)。


The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).


  1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
  2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
  3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
  4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
  5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
  6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
  7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
  8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
  9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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