Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Gab1 (Tyr627) Antibody #3231

GRB-associated-binder   GRB-associated-binder-1   GRB-associated-binding-protein   GRB2-associated-binder   GRB2-associated-binding-protein-1  

No. Size Price
3231S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3231 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 110 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Hamster,

Specificity / Sensitivity

Phospho-Gab1 (Tyr627) Antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 627. The antibody may cross-react with phosphorylated Gab2 or Gab3, or with activated receptor tyrosine kinases (RTKs).

Phospho-Gab1 (Tyr627) Antibody能够检测Tyr627磷酸化的内源性Gab1蛋白。抗体也可以与磷酸化的Gab2及磷酸化的Gab3或者活化的受体酪氨酸激酶(RTKs)交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源Gab1蛋白Tyr627周围的序列相对应的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells, untreated or treated with In1B for the indicated times, using Phospho-Gab1 (Tyr627) Antibody (upper) or Gab1 Antibody #3232 (lower).

Western blot 检测分析HEK293细胞提取物,未处理的和用 In1B处理特定时间的;使用的抗体是 Phospho-Gab1 (Tyr627)Antibody(上图)和Gab1 Antibody #3232 (下图)。

Background

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).

Grb相关结合因子(Gab)家族是一类由多种受体酪氨酸激酶(RTKs),如EGFR,HGFR,胰岛素受体,细胞因子受体和B细胞抗原受体招募的接头蛋白。RTKs受到它们的同源配体刺激后,Gab被招募到细胞膜上,接着其被磷酸化,并起到支架的作用(1-4)。Gab1蛋白存在多个酪氨酸磷酸化位点(5)。酪氨酸(472位点)磷酸化可以调节其与PI3K激酶p85的结合(6,7)。Gab1酪氨酸(307,373和407位点)的磷酸化可以调节Gab1与PLCγ的互作(8)。酪氨酸(627和659位点)磷酸化是Gab1结合并活化蛋白酪氨酸磷酸酶SHP2所必需的(6,9)。

  1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
  2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
  3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
  4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
  5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
  6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
  7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
  8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
  9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

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