Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Ret (Tyr905) Antibody #3221

No. Size Price
3221S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3221 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,D. melanogaster, Endogenous 175 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Ret (Tyr905) Antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 905.

磷酸化Ret (Tyr905) 抗体仅在Ret蛋白的tyrosine 905位点磷酸化时能检测到内源的Ret蛋白的表达。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用合成的磷酸化多肽免疫动物得到,该多肽是根据人的Ret蛋白Tyr905附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-Ret (His658-Asp1110) and extracts from the TT human medullary thyroid carcinoma cell line using Phospho-Ret (Tyr905) Antibody. 用Phospho-Ret (Tyr905) Antibody对以下样品进行免疫印迹检测:重组的人的GST-Ret (His658-Asp1110),TT human medullary thyroid carcinoma细胞系的提取物。

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-Ret (His658-Asp1110), untreated or λ phosphatase-treated, using Phospho-Ret (Tyr905) Antibody (upper) and Ret Antibody #3220 (lower). 用Phospho-Ret (Tyr905) Antibody (上) 和 Ret Antibody #3220 (下)对未处理的和经过λ 磷酸酶处理的重组的人的GST-Ret (His658-Asp1110)进行免疫印迹检测。

Background

The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity (5).

Ret原癌基因(c-Ret)是一个多功能的酪氨酸激酶受体复合物,它和其它膜结合的GDNF家族的配体结合型受体共同发挥作用(1)。与Ret受体结合的配体包括神经胶质细胞来源的神经营养因子(GDNF)和它的同类物neurturin、 persephin 和 artemin (2-4)。在相应的Ret 基因的改变会引起乳突状甲状腺癌、多发性内分泌肿瘤(2A和2B型),家族性髓样甲状腺癌和先天性巨结肠病(1,3)。位于Ret激酶区的Tyr905位点对于Ret的催化功能和生物学功能十分重要。替换Tyr905的Phe能显著抑制Ret的自磷酸化活性(5)。

  1. Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25.
  2. Takahashi, M. et al. (1989) Oncogene 4, 805-6.
  3. Manié, S. et al. (2001) Trends Genet 17, 580-9.
  4. Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
  5. Iwashita, T. et al. (1999) Oncogene 18, 3919-22.
  6. Plaza-Menacho, I. et al. (2014) Mol Cell 53, 738-51.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications