Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Ret (Tyr905) Antibody #3221

No. Size Price
3221S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3221 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,D. melanogaster, Endogenous 175 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Ret (Tyr905) Antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 905.

磷酸化Ret (Tyr905) 抗体仅在Ret蛋白的tyrosine 905位点磷酸化时能检测到内源的Ret蛋白的表达。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用合成的磷酸化多肽免疫动物得到,该多肽是根据人的Ret蛋白Tyr905附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-Ret (His658-Asp1110) and extracts from the TT human medullary thyroid carcinoma cell line using Phospho-Ret (Tyr905) Antibody. 用Phospho-Ret (Tyr905) Antibody对以下样品进行免疫印迹检测:重组的人的GST-Ret (His658-Asp1110),TT human medullary thyroid carcinoma细胞系的提取物。

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-Ret (His658-Asp1110), untreated or λ phosphatase-treated, using Phospho-Ret (Tyr905) Antibody (upper) and Ret Antibody #3220 (lower). 用Phospho-Ret (Tyr905) Antibody (上) 和 Ret Antibody #3220 (下)对未处理的和经过λ 磷酸酶处理的重组的人的GST-Ret (His658-Asp1110)进行免疫印迹检测。


The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity (5).

Ret原癌基因(c-Ret)是一个多功能的酪氨酸激酶受体复合物,它和其它膜结合的GDNF家族的配体结合型受体共同发挥作用(1)。与Ret受体结合的配体包括神经胶质细胞来源的神经营养因子(GDNF)和它的同类物neurturin、 persephin 和 artemin (2-4)。在相应的Ret 基因的改变会引起乳突状甲状腺癌、多发性内分泌肿瘤(2A和2B型),家族性髓样甲状腺癌和先天性巨结肠病(1,3)。位于Ret激酶区的Tyr905位点对于Ret的催化功能和生物学功能十分重要。替换Tyr905的Phe能显著抑制Ret的自磷酸化活性(5)。

  1. Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25.
  2. Takahashi, M. et al. (1989) Oncogene 4, 805-6.
  3. Manié, S. et al. (2001) Trends Genet 17, 580-9.
  4. Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
  5. Iwashita, T. et al. (1999) Oncogene 18, 3919-22.
  6. Plaza-Menacho, I. et al. (2014) Mol Cell 53, 738-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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