Cell Signaling Technology

Product Pathways - Metabolism

Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203

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No. Size Price
3203S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3203T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3203 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 180 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb detects endogenous levels of IRS-1 only when phosphorylated at Ser612.

Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb兔单抗用于检测内源性在612位丝氨酸磷酸化的IRS-1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser612 of mouse IRS-1.

单克隆抗体通过用对应于人源IRS-1蛋白Ser612序列的合成肽免疫动物制备而得。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from CHO IR/IRS-1 cells, untreated or treated with insulin, using Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.

Wetern blot检测CHO IR/IRS-1细胞提取物,细胞未处理或用胰岛素处理,所用抗体为Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb兔单抗 (上中)和IRS-1 Antibody #2382 (下)。中间道事先用小肠磷酸酶进行处理。

Background

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

胰岛素受体底物1(IRS-1)是胰岛素受体激酶的一类主要底物(1)。 IRS-1包含多个酪氨酸磷酸化位点,可以作为含SH2结构域蛋白的停靠位点,这些蛋白调控着胰岛素的代谢和促生长功能(2-4) 。IRS-1还包含着起过30个潜在的丝氨酸/苏氨酸磷酸化位点,307位丝氨酸可以被JNK(5)和IKK(6)磷酸化,789位的丝氨酸可以被SIK-2(是AMPK家族成员)磷酸化(7)。PKC和mTOR途径可以分别磷酸化IRS-1的612位丝氨酸和636/639位丝氨酸(8,9)。PKCθ可以介导IRS-2在1101位丝氨酸位的磷酸化,此位点的磷酸化会导致细胞胰岛素信号通路的抑制,因此它可能在某些肥胖模型的胰岛素抵抗中起着某些作用(10)。

Phosphorylation of IRS-1 at Ser612 by MAPK downregulates insulin signaling and may be part of a response to high glucose/glucosamine levels (11).

IRS-1的612位丝氨酸被MAPK磷酸化后会下调胰岛素信号,这可能是对高葡萄糖/葡萄糖胺比值的一种反应。

  1. Sun, X.J. et al. (1991) Nature 352, 73-77.
  2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
  3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
  4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
  5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
  6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
  7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
  8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
  9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
  10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
  11. Andreozzi, F. et al. (2004) Endocrinology 145, 2845-57.

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U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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