Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

eNOS (D9A5L) Rabbit mAb #32027


No. Size Price
32027S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
32027 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Bovine, Endogenous 140 Rabbit IgG
IP 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

eNOS (D9A5L) Rabbit mAb recognizes endogenous levels of total eNOS protein. This antibody does not cross-react with overexpressed iNOS nor nNOS.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1190 of human eNOS protein.



Confocal immunofluorescent analysis of HUVE (left) and HeLa (right) cells using eNOS (D9A5L) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC, HeLa and EA.hy.926 cells using eNOS (D9A5L) Rabbit mAb (upper) or β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).


Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

  1. Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24.
  2. Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74.
  3. Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9.
  4. Dimmeler, S. et al. (1999) Nature 399, 601-5.
  5. Fulton, D. et al. (1999) Nature 399, 597-601.
  6. Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91.
  7. Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24.

Application References

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