Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179


No. Size Price
3179L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3179S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3179 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 170 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-PERK (Thr980) (16F8) Rabbit mAb detects endogenous levels of PERK phosphorylated at Thr980.

Phospho-PERK (Thr980) (16F8) Rabbit mAb兔单抗可识别内源性的Thr980磷酸化的PERK。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK.


Western Blotting

Western Blotting

Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.

对AR42J细胞(未处理(-),或1uM thapsigargin (Tg) (+) 处理20分钟),使用Phospho-PERK (Thr980) (16F8) Rabbit mAb兔单抗进行Western blot分析。


PERK (protein kinase-like endoplasmic reticulum kinase) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

蛋白激酶类似的内质网激酶(PERK)是一种eIF2α激酶并且为一种定位在endoplasmic reticulum (ER) 膜上的跨膜蛋白,能够传导ER应激信号从而抑制翻译(1-3)。ER应激会提高PERK的活性,然后使eIF2α磷酸化从而增加翻译水平的降低。研究证实PERK缺失的小鼠在出生后的几周内其胰岛β细胞会出现缺陷,这也暗示着PERK介导的翻译控制在ER应激时保护分泌细胞的重要作用(4)。ER应激时PERK的活化与其胞内激酶结构域的自身磷酸化相关联(1-3)。PERK Thr980位点的磷酸化是其自身活化状态的一个标志。

  1. Harding, H. et al. (1999) Nature 397, 271-274.
  2. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
  3. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :