Cell Signaling Technology

Product Pathways - Innate Immunity

cGAS (D3O8O) Rabbit mAb (Mouse Specific) #31659

No. Size Price
31659S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
31659 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 62 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

cGAS (D3O8O) Rabbit mAb (Mouse Specific) recognizes endogenous levels of mouse cGAS protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala66 of mouse cGAS protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3, Neuro-2a, and C2C12 cells using cGAS (D3O8O) Rabbit mAb (Mouse Specific).

Western Blotting

Western Blotting

Western Blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse cGAS protein (mcGAS-Myc/DDK; +), using cGAS (D3O8O) Rabbit mAb (Mouse Specific).

IP

IP

Immunoprecipitation of cGAS from C2C12 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is cGAS (D3O8O) Rabbit mAb (Mouse Specific). Western blot analysis was performed using cGAS (D3O8O) Rabbit mAb (Mouse Specific).

Background

Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5).

  1. Sun, L. et al. (2013) Science 339, 786-91.
  2. Wu, J. et al. (2013) Science 339, 826-30.
  3. Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
  4. Zhong, B. et al. (2008) Immunity 29, 538-50.
  5. Ishikawa, H. et al. (2009) Nature 461, 788-92.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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