Cell Signaling Technology

Product Pathways - NF-kB Signaling

Pellino-1 (D2Z4F) Rabbit mAb #31474

Peli1   Pellino   Pellino 1   Pellino1  

No. Size Price
31474S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
31474 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 47 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Pellino-1 (D2Z4F) Rabbit mAb recognizes endogenous levels of total Pellino-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala403 of human Pellino-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pellino-1 (D2Z4F) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of 293T cells, mock tranfected (-) or transfected with a construct expressing full-length human Myc/DDK-tagged Pellino-1 (hPellino-1-Myc/DDK; +), using Pellino-1 (D2Z4F) Rabbit mAb.

Background

Pellino-1 (PELI1) is an E3 ubiquitin ligase that regulates activation of various immune pathways in different contexts and cell types (1). Pellino-1 controls NFκB activation downstream of the interleukin-1 receptor and TLRs through association with and ubiquitination of IRAK1 (1-4). Pellino-1 was also demonstrated to regulate the NFκB pathway in T cells by targeting c-Rel for degradation, thereby enabling suppression by regulatory T cells, maintaining peripheral tolerance, and preventing autoimmunity in mice (5). Finally, in experimental autoimmune encephalomyelitis (EAE), the mouse model of multiple sclerosis, Pellino-1 contributes to inflammation by promoting degradation of Traf3 leading to induction of chemokines and proinflammatory cytokines (6).

  1. Schauvliege, R. et al. (2006) FEBS Lett 580, 4697-702.
  2. Jiang, Z. et al. (2003) J Biol Chem 278, 10952-6.
  3. Butler, M.P. et al. (2007) J Biol Chem 282, 29729-37.
  4. Murphy, M. et al. (2015) J Biol Chem 290, 19218-32.
  5. Chang, M. et al. (2011) Nat Immunol 12, 1002-9.
  6. Xiao, Y. et al. (2013) Nat Med 19, 595-602.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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