Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Ezh2 (AC22) Mouse mAb #3147

histone methyltransferase   HMT  

No. Size Price
3147S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
3147 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 98 Mouse IgG1
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Ezh2 (AC22) Mouse mAb detects endogenous levels of total Ezh2 protein.

Ezh2 (AC22) Mouse mAb鼠单抗能够检测内源性Ezh2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with Ezh2 recombinant protein containing human Ezh2 residues 351-453.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ezh2 (AC22) Mouse mAb.

使用Ezh2 (AC22) Mouse mAb鼠单抗,免疫印迹(Western blot)分析不同细胞系中Ezh2 (AC22)的蛋白水平。



Confocal immunofluorescent analysis of HeLa cells using Ezh2 (AC22) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

使用Ezh2 (AC22) Mouse mAb 鼠单抗(绿色)标记,共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。


The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

polycomb group (PcG)蛋白是涉及维持数个发育调节基因的沉默水平,并且有助于维持细胞身份(cell identity)、细胞周期调节和肿瘤发生(1,2)。zeste homolog 2 (Ezh2)的增强子是一个该蛋白家族成员,它包含四种保守区域包括domain I、domain II、一个半胱氨酸富裕的氨基酸延伸序列和羧基端SET domain (3)。SET domain已经证明与histone methyltransferase (HMTase)活性有关联。此外,哺乳类Ezh2是一个组蛋白去乙酰酶复合物的成员之一,它在基因沉默中有功能,并且在染色质结构水平起着作用(4)。在体内,Ezh2复合物使histone H3在Lys9和27位点甲基化,这被认为是涉及到调节转录因子到特异的靶位点(5)。Ezh2蛋白在不同肿瘤形式中被下调,并且具有主要效应因子和肿瘤发生的调节因子的作用已经变成一个越来越感兴趣的课题(6)。

  1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
  2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
  3. Chen, H. et al. (1996) Genomics 38, 30-7.
  4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
  5. Müller, J. et al. (2002) Cell 111, 197-208.
  6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

Application References

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