Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Ezrin (Tyr353) Antibody #3144

No. Size Price
3144S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3144 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

Phospho-Ezrin (Tyr353) Antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353. The antibody does not cross-react with phosphorylated Moesin or Radixin.

Phospho-Ezrin (Tyr353) 仅识别tyrosine 353磷酸化的内源性ezrin蛋白。该抗体不能与磷酸化的Moesin或Radixin蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr353 of human Ezrin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-treated, using Phospho-Ezrin (Tyr353) Antibody (upper) or Ezrin/Radixin/Moesin Antibody #3142 (lower). 使用Phospho-Ezrin (Tyr353) Antibody (上图)或Ezrin/Radixin/Moesin Antibody #3142 (下图),免疫印迹(Western Blot)分析A431细胞中Phospho-Ezrin (Tyr353)和Ezrin/Radixin/Moesin蛋白水平,细胞分为未处理组和EGF处理组。


The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

ezrin, radixin, 和moesin (ERM)蛋白功能作为细胞质膜和肌动蛋白细胞骨架的连接者,并且该蛋白涉及细胞粘附、胞膜边缘波动(membrane ruffling)和微绒毛形成 (1)。ERM蛋白参与分子内或分子间的氨基和羧基端结构域的相互作用,该蛋白是以非活性的细胞基质单体或二聚体存在 (2)。ERM蛋白在羧基端残基(ezrin蛋白Thr567位点、radixin蛋白Thr564位点、moesin蛋白Thr558 位点) 磷酸化可干扰氨基端和羧基端的关联,这可能在调节ERM蛋白的形成和功能中起着重要作用 (3,4)。ezrin蛋白Thr567位点磷酸化对于细胞骨架的重组和致癌基因诱导的转化可能起着关键角色(5)。基于生长因子刺激,Ezrin蛋白也在酪氨酸残基发生磷酸化。在上皮细胞分化期间,ezrin蛋白Tyr353位点的磷酸化传递一个生存信号(6)。

  1. Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.
  2. Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.
  3. Matsui, T. et al. (1998) J Cell Biol 140, 647-57.
  4. Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.
  5. Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.
  6. Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.

Application References

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